2. Academic Validation
  3. High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization

High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization

  • Sci Rep. 2025 Jul 1;15(1):20888. doi: 10.1038/s41598-025-04434-1.
Zihao Zhang 1,  Jian Han 1 2,  Ke Wei 1,  Shixi Chen 3 4
Affiliations

Affiliations

  • 1 College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, Fujian, China.
  • 2 Key Laboratory of Fish Applied Biology and Aquaculture in North China, College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, Liaoning, China.
  • 3 College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, Fujian, China. chenshixi@jmu.edu.cn.
  • 4 Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, Xiamen, 361021, Fujian, China. chenshixi@jmu.edu.cn.
PMID: 40593938 DOI: 10.1038/s41598-025-04434-1
Abstract

Chromatophores provide an excellent model to study organelle transport as they specialize in the translocation of pigment granules in response to defined environmental signals. Our previous study revealed low-intensity light-induced xanthosomes aggregation is directly mediated by Opsin 3 in xanthophores of large yellow croaker (Larimichthys crocea). Herein, we report an incidental observation of prolonged (more than 40 min) high-intensity white light (HIWL) (10,000 lux, 3.86 mW/cm2) exposure induce xanthosomes dispersion. After dispersion by HIWL exposure, the xanthosomes remains the ability to aggregate under low-intensity light conditions. Both light-emitting diode (LED) sources at different wavelengths (blue light, λmax = 480 nm; red light, λmax = 686 nm) with identical brightness (10,000 lux) but varying absolute irradiance levels (3.86-22.2 mW/cm2) can induce xanthosome dispersion. Ex vivo illumination and pharmacological experiments on xanthophores revealed that HIWL-induced xanthosomes dispersion is irrelevant to signaling pathways typically associated with xanthosome movement, but mediated by microtubule depolymerization, which is due to extraordinary high level of intracellular CA2+ released from IP3R Calcium Channel in endoplasmic reticulum. Interestingly, such a mechanism was also presented in HeLa and HEK293T cells exposed to HIWL. In summary, our results expand our understanding of the impact of high-intensity light on intracellular organelle transport and Cytoskeleton.

Keywords

Ca2+; Chromatophore; Fish; High-intensity light; Light; Microtubule.

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