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  3. Gut microbiota-bile acid crosstalk contributes to calcium oxalate nephropathy through Hsp90α-mediated ferroptosis

Gut microbiota-bile acid crosstalk contributes to calcium oxalate nephropathy through Hsp90α-mediated ferroptosis

  • Cell Rep. 2025 Jul 22;44(7):115936. doi: 10.1016/j.celrep.2025.115936.
Linhu Liu 1,  Yucheng Ma 1,  Zhongyu Jian 1,  Banghua Liao 1,  Ya Li 1,  Lede Lin 1,  Menghua Wang 1,  Jiawei Chen 1,  Jingwen Wei 1,  Mengzhu Yang 1,  Yu Liu 1,  Yiqiong Yuan 2,  Jun Wen 1,  Liyuan Xiang 1,  Shiqian Qi 2,  Xi Jin 3,  Kunjie Wang 4
Affiliations

Affiliations

  • 1 Department of Urology/Institute of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
  • 2 Department of Urology/Institute of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China; Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and National Collaborative Innovation Center, Chengdu, Sichuan 610041, P.R. China.
  • 3 Department of Urology/Institute of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. Electronic address: jinxi@wchscu.cn.
  • 4 Department of Urology/Institute of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China. Electronic address: wangkj@scu.edu.cn.
PMID: 40591459 DOI: 10.1016/j.celrep.2025.115936
Abstract

The gut microbiota and its metabolites have been implicated in calcium oxalate (CaOx) nephrolithiasis, while the precise mechanism remains unclear. We report that the gut microbiota dysbiosis in patients with CaOx nephrolithiasis results in an elevated level of deoxycholic acid (DCA) and diminished presence of Faecalibacterium prausnitzii. DCA correlates positively with urinary oxalate excretion and stone burden and inversely with F. prausnitzii abundance. Administration of F. prausnitzii to high-oxalate-diet mice alleviates renal CaOx crystal deposition by reducing DCA and secondary bile acid production. DCA impairs intestinal oxalate catabolism, enhances CaOx crystal adhesion to renal tubular epithelial cells via Hsp90α upregulation, activates the Hsp90α/Nrf2/HO-1 pathway, and induces Gpx4 degradation by Hsp90α interaction and ubiquitination, resulting in ferrous ion accumulation, lipid peroxidation, and Ferroptosis to facilitate renal injury and CaOx crystal deposition. These data highlight the important role of gut microbiota-bile acid crosstalk in the pathogenesis of CaOx nephrolithiasis.

Keywords

CP: Metabolism; CP: Microbiology; bile acids; calcium oxalate nephrolithiasis; crystal adhesion; ferroptosis; gut microbiota; heat shock protein 90α; kidney stone.

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