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  3. Targeting Annexin A2 to reactivate tumor-associated antigens presentation and relieve immune tolerance in liver cancer

Targeting Annexin A2 to reactivate tumor-associated antigens presentation and relieve immune tolerance in liver cancer

  • J Immunother Cancer. 2025 Jun 26;13(6):e011716. doi: 10.1136/jitc-2025-011716.
Yuxiao Tang # 1,  Jianxin Yang # 1,  Qicong Shen # 2,  Zelong Gao 1,  Mengpu Wu 1,  Chenghua Wu 3,  Jicong Du 4,  Min Li 1,  Changquan Ling 5,  Feng Lu 6,  Yifeng Chai 6,  Xin Dong 7,  Jianxin Qian 3,  Chenqi Li 1 8,  Feng Xie 8,  Zhenhong Guo # 2,  Hui Shen 9,  Dongyao Wang 10 6
Affiliations

Affiliations

  • 1 Department of Nutrition, Key Laboratory of Biological Defense, Ministry of Education, Second Military Medical University, Shanghai, Shanghai, China.
  • 2 National Key Laboratory of Medical Immunology, Institute of Immunology, Second Military Medical University, Shanghai, China.
  • 3 Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  • 4 Department of Radiation Medicine, Second Military Medical University, Shanghai, China.
  • 5 Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai, China.
  • 6 Department of Pharmaceutical Analysis, Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University, Shanghai, China.
  • 7 School of medicine, Shanghai University, Shanghai, China.
  • 8 Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
  • 9 Department of Nutrition, Key Laboratory of Biological Defense, Ministry of Education, Second Military Medical University, Shanghai, Shanghai, China wangdongyao@smmu.edu.cn shenhui@smmu.edu.cn.
  • 10 National Key Laboratory of Medical Immunology, Institute of Immunology, Second Military Medical University, Shanghai, China wangdongyao@smmu.edu.cn shenhui@smmu.edu.cn.
  • # Contributed equally.
PMID: 40571481 DOI: 10.1136/jitc-2025-011716
Abstract

Background: Tumor cells manipulate the tumor-associated antigens presentation to escape immune surveillance; however, the molecular mechanism is not exactly clear and the measure to intervene is missing.

Methods: Annexin A2 was knockout by the CRISPR-Cas9 or blocked by the small-molecule matrine, PY60, and hexapeptide. Chemically and genetically induced primary liver Cancer models, and the orthotopically implanted liver tumor model were used. Tumor immune environment was analyzed by single-cell Sequencing. Annexin A2-interacted proteins and tumor-associated antigens were identified by co-immunoprecipitation coupled with liquid chromatography with tandem mass spectrometry. Tumor cells killing effects were evaluated by co-culture of tumor cells and CD8+ T cells.

Results: Targeting Annexin A2 effectively suppressed the progression of liver Cancer. The immunosuppressive microenvironment was improved by Annexin A2 inhibition in tumor tissues. The CD8+ T cells were increased and activated by targeting Annexin A2. Mechanistically, targeting Annexin A2 inhibited its combination with HSP90. The HSP90-mediated tumor-associated antigens presentation was recovered, and the major histocompatibility complex I-presented short peptides were changed, increasing the tumor cells killing by CD8+ T cells. Interestingly, Annexin A2 was increased in liver Cancer tissues and the overall survival was significantly reduced in patients with high expression. However, Annexin A2 was positively correlated with immune cell infiltration in liver Cancer, implying that Annexin A2 was used by tumor cells for immune escape and immunotherapy resistance. Hence, we further confirmed that blocking Annexin A2 increased the therapeutic effects of anti-programmed cell death protein-1 both in vitro and in vivo.

Conclusions: Taken together, our results identified the role of Annexin A2 in the tumor-associated antigens presentation and immune evasion, which could be an actionable target in Cancer Immunotherapy.

Keywords

Hepatocellular Carcinoma; Immune modulatory; Immunotherapy.

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