2. Academic Validation
  3. Investigating the effects of dendrobine on fibrosis and inflammation in orbital fibroblasts of thyroid-associated ophthalmopathy via network pharmacology, molecular docking, and transcriptomics

Investigating the effects of dendrobine on fibrosis and inflammation in orbital fibroblasts of thyroid-associated ophthalmopathy via network pharmacology, molecular docking, and transcriptomics

  • Eur J Pharmacol. 2025 Sep 5:1002:177871. doi: 10.1016/j.ejphar.2025.177871.
Meng Xie 1,  Bowen Wang 2,  Jin Chen 2,  Yiyan Wang 2,  Yan Rong 3,  Zixuan Su 2,  Fagang Jiang 4,  Xinghua Wang 5
Affiliations

Affiliations

  • 1 Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China; Department of Ophthalmology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, China.
  • 2 Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
  • 3 Department of Ophthalmology, Hubei Provincial Traditional Chinese Medicine Hospital, Wuhan 430061, Hubei Province, China.
  • 4 Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. Electronic address: fgjiang@hust.edu.cn.
  • 5 Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. Electronic address: xinghua_wang@hust.edu.cn.
PMID: 40544935 DOI: 10.1016/j.ejphar.2025.177871
Abstract

This study aims to evaluate the effects and mechanisms of dendrobine against thyroid-associated ophthalmopathy (TAO). Potential targets of dendrobine and TAO were selected to construct a protein-protein interaction network, elucidating the potential mechanisms of dendrobine against TAO. The potential mechanisms systematically were verified by molecular docking, transcriptomics and in vitro experiments. Orbital fibroblasts (OFs) were isolated from orbital decompression-derived fat tissue, and the effects of dendrobine were studied in transforming growth factor-beta 1 (TGF-β1)-induced fibrosis models and interleukin-1β (IL-1β)-induced inflammation models. The results showed that dendrobine inhibited the migration, fibrosis and levels of inflammatory factors in TAO OFs. Network pharmacology and transcriptomics studies revealed the mechanisms of dendrobine, while in vitro experiments confirmed it alleviates TGF-β1-induced fibrosis in TAO OFs by inhibiting Akt phosphorylation.

Keywords

AKT; Dendrobine; Fibrosis; Molecular docking; Network pharmacology; Thyroid-associated ophthalmopathy; Transcriptomics.

Figures
Products
Inhibitors & Agonists
Other Products