2. Academic Validation
  3. Gasdermin E in glioblastoma -pyroptosis resistance and tumor-promoting functions

Gasdermin E in glioblastoma -pyroptosis resistance and tumor-promoting functions

  • Cell Death Discov. 2025 Jun 21;11(1):284. doi: 10.1038/s41420-025-02572-z.
Ege Solel # 1,  Egil Brudvik # 1,  Lars Andreas Rømo Ystaas 2,  Yahaya A Yabo 3,  Emma Rigg 1,  Romi Roy Choudhury 1,  Halala Sdik Saed 1,  Dieter Henrik Heiland 3 4 5 6,  Rolf Bjerkvig 1,  Jubayer Hossain 1 7,  Hrvoje Miletic 8 9
Affiliations

Affiliations

  • 1 Department of Biomedicine, University of Bergen, Bergen, Norway.
  • 2 Institute for Clinical Medicine, University of Oslo, Oslo, Norway.
  • 3 Department of Neurosurgery, Medical Center, Faculty of Medicine, Erlangen University, Erlangen, Germany.
  • 4 Comprehensive Cancer Center Freiburg (CCCF), Medical Center, University of Freiburg, Freiburg, Germany.
  • 5 German Cancer Consortium (DKTK) partner site Freiburg, Freiburg, Germany.
  • 6 Department of Neurological Surgery, Lou and Jean Malnati Brain Tumor Institute, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
  • 7 Faculty of Nursing and Health Sciences, Nord University, Namsos, Norway.
  • 8 Department of Biomedicine, University of Bergen, Bergen, Norway. Hrvoje.miletic@uib.no.
  • 9 Department of Pathology, Haukeland University Hospital, Bergen, Norway. Hrvoje.miletic@uib.no.
  • # Contributed equally.
PMID: 40544161 DOI: 10.1038/s41420-025-02572-z
Abstract

Treatment of glioblastoma (GB), the most common and most aggressive malignant brain tumor, has made little progress over the past two decades. Despite extensive research on Apoptosis and Autophagy, necrotic cell death mechanisms like Pyroptosis, which have the potential to stimulate anti-tumor immune responses, remain largely underexplored in GB. Here, we investigated whether Gasdermin E (GSDME)-mediated Pyroptosis can be induced in GB by employing the drug raptinal, an inducer of cytochrome c release. Using human patient-derived and mouse GB cell lines, we showed that raptinal promotes GSMDE cleavage. However, although a strong pyroptotic response was observed in mouse cell lines, it was weak in human cell lines. This resistance was partially reversed by the calcium chelator BAPTA-AM, indicating that membrane repair mechanisms may counteract the pyroptotic response. Gsdme knockout (KO) in mouse GB cells unexpectedly prolonged the survival of immunocompetent mice, demonstrating a tumor-promoting role of GSDME independent of its pyroptotic function. Analysis of the immune microenvironment revealed that Gsdme KO promoted infiltration of T cells, which was confirmed by spatial transcriptomic analysis of GB patient samples. In addition, Gsdme/GSMDE KO reduced the invasive capacity of mouse/human GB cells. In conclusion, active membrane repair mechanisms may impair the pyroptotic efficacy in GB. GSDME has a tumor-promoting role in GB by suppressing T cell infiltration and increasing tumor cell invasion.

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