Bioinformatics and experiments reveal the hub genes of age-related hearing loss and the mechanism of PLK1 silencing in the protection of aging cochlear hair cells

Objective: We aim to identify the hub genes and explore molecular mechanisms underlying age-related hearing loss (ARHL) through multi-omics approaches and experimental validation.

Methods: Differentially expressed genes (DEGs) were screened from the GSE6045 dataset for functional enrichment analysis, protein-protein interaction (PPI) network analysis, and gene set enrichment analysis (GSEA), along with an investigation into the immune microenvironment in ARHL. Hub genes were assessed using the receiver operating characteristic (ROC) curves and further validated using GSE49543 datasets and ARHL mouse models induced by D-galactose (D-gal). The polo-like kinase 1 (PLK1) knockdown in D-gal-induced HEI-OC1 cells was conducted to explore its role in Apoptosis and oxidative stress.

Results: Hub genes (MCM6, WDHD1, ATR, PLK1, and RRS1) were identified with high prediction values for ARHL (The value of area under the curve [AUC] > 0.7). There were significant differences in immune cells between young and old samples with hearing loss. ARHL mice exhibited elevated auditory thresholds, pathological damage, and cell Apoptosis of cochlear tissues. MCM6, WDHD1, and PLK1 were upregulated in cochlear tissues of ARHL mice, whereas ATR and RRS1 were downregulated. The ROC analysis highlighted PLK1 as a potential diagnostic marker (AUC > 0.9). PLK1 silencing reduced Reactive Oxygen Species and Apoptosis in D-gal-treated HEI-OC1 cells.

Conclusion: MCM6, WDHD1, ATR, PLK1, and RRS1 were hub genes of ARHL. PLK1 knockdown mitigates oxidative damage and Apoptosis of D-gal-induced HEI-OC1 cells, offering insights for ARHL intervention.

Age-related hearing loss; Differentially expressed genes; HEI-OC1 cells; Hub genes, Polo-like kinase 1.