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  3. Abscisic acid enhances DNA damage response through the nuclear shuttling of clathrin light chain 2 in plant cells

Abscisic acid enhances DNA damage response through the nuclear shuttling of clathrin light chain 2 in plant cells

  • Sci Adv. 2025 Jun 13;11(24):eadt2842. doi: 10.1126/sciadv.adt2842.
Jieming Jiang 1,  Qiuhua Zhu 2,  Yang Li 1,  Jiayuan Wu 1,  Chenghuan Zou 1,  Hongbo Li 1,  Xiaohui Lei 1,  Fangyuan Zhang 1,  Yujie Lin 1,  Rui Cao 1,  Ying Cao 1,  Jiawang Mo 1,  Jianbin Lai 1,  Jiangzhe Zhao 3,  Chao Wang 2,  Chengwei Yang 1
Affiliations

Affiliations

  • 1 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China.
  • 2 College of Life Sciences, Shaoxing University, Shaoxing 312000, China.
  • 3 College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
PMID: 40512838 DOI: 10.1126/sciadv.adt2842
Abstract

DNA damage arises from various environmental stresses, and ABA is well known for its roles in plant stress resistance. However, its function in plant DNA damage tolerance remains unclear. In this study, we showed that ABA supplementation significantly enhances plant tolerance to DNA-damaging treatments. SnRK2.2 and SnRK2.3 kinases in the ABA signaling pathway are pivotal in this process. These kinases interact with clathrin light chain 2 (CLC2), facilitating its phosphorylation and nuclear translocation in response to Zeocin and ABA treatment. In the nucleus, CLC2 interacts with ADA2b, an adaptor protein crucial for recruiting SMC5/6 complex to the double-strand break (DSB) sites. The enhanced nuclear localization of CLC2 is essential for the accurate localization of ADA2b at DSB sites. Collectively, our study uncovers that ABA enhances plant DNA damage tolerance with a distinct function of CLC2 in genomic stability maintaining, thereby improving our understanding of DNA damage tolerance mechanisms in Plants.

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