2. Academic Validation
  3. Effects of Chitosan on Drug Load and Release for Cisplatin-Hydroxyapatite-Gelatin Composite Microspheres

Effects of Chitosan on Drug Load and Release for Cisplatin-Hydroxyapatite-Gelatin Composite Microspheres

  • Polymers (Basel). 2025 May 27;17(11):1485. doi: 10.3390/polym17111485.
Meng-Ying Wu 1 2 3 4 5,  I-Fang Kao 1,  Shiow-Kang Yen 1
Affiliations

Affiliations

  • 1 Department of Materials Science and Engineering, National Chung Hsing University, Taichung 402, Taiwan.
  • 2 Department of Orthopedics, Taichung Armed Forces General Hospital, Taichung 404, Taiwan.
  • 3 Department of Gerontological Health Care, Central Taiwan University of Science and Technology, Taichung 406, Taiwan.
  • 4 Department of Natural Biotechnology, Nanhua University, Chiayi 622, Taiwan.
  • 5 Department of Dental Technology and Material Science, Central Taiwan University of Science and Technology, Taichung 406, Taiwan.
PMID: 40508728 DOI: 10.3390/polym17111485
Abstract

Cisplatin, a widely used chemotherapeutic agent, is limited by its poor bioavailability, rapid systemic clearance, and severe side effects. To overcome these limitations, hydroxyapatite-gelatin composite microspheres were developed to improve drug entrapment efficiency (DEE) and provide sustained drug release. Various formulations were prepared by incorporating chitosan either by mixing once or through a sequential coating strategy. By adjusting the loading procedure, the DEE increased from 58% to 99%. The composite microsphere effectively controlled the total drug release duration, extending it from one month to over 5 months. Moreover, the MTT assay demonstrated that all samples effectively inhibited cell growth, with cell viability reduced to less than 20% after 2 weeks of experimentation. These findings demonstrate that the sequential chitosan coating method offers superior drug entrapment and prolonged release compared to mixing chitosan once, exhibiting its potential as a sustained drug delivery system for Cancer treatment.

Keywords

chitosan; cisplatin; cone-like pores; drug entrapment efficiency; hydroxyapatite–gelatin microspheres; release duration.

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