2. Academic Validation
  3. Chicago Sky Blue 6B modulates MAPK/NF-kB pathway in rat knee osteoarthritis

Chicago Sky Blue 6B modulates MAPK/NF-kB pathway in rat knee osteoarthritis

  • Naunyn Schmiedebergs Arch Pharmacol. 2025 Jun 12. doi: 10.1007/s00210-025-04353-y.
Yejin Zhang 1 2,  Weiqing Li 1 2,  Yu Bai 1 2,  Hehuan Lai 1 2,  Jiawei Fang 1 2,  Lin Ye 3 4,  Dengwei He 5 6,  Zhenzhong Chen 7 8
Affiliations

Affiliations

  • 1 Department of Orthopedics Surgery, Lishui Hospital, Zhejiang University School of Medicine, Lishui, China.
  • 2 Department of Orthopaedic Surgery, Lishui Central Hospital and Fifth Affiliated Hospital of Wenzhou Medical College, 289 Kuocang Road, Lishui, 323000, Zhejiang, China.
  • 3 Department of Orthopedics Surgery, Lishui Hospital, Zhejiang University School of Medicine, Lishui, China. yunheyelin@163.com.
  • 4 Department of Orthopaedic Surgery, Lishui Central Hospital and Fifth Affiliated Hospital of Wenzhou Medical College, 289 Kuocang Road, Lishui, 323000, Zhejiang, China. yunheyelin@163.com.
  • 5 Department of Orthopedics Surgery, Lishui Hospital, Zhejiang University School of Medicine, Lishui, China. hedw_spine@163.com.
  • 6 Department of Orthopaedic Surgery, Lishui Central Hospital and Fifth Affiliated Hospital of Wenzhou Medical College, 289 Kuocang Road, Lishui, 323000, Zhejiang, China. hedw_spine@163.com.
  • 7 Department of Orthopedics Surgery, Lishui Hospital, Zhejiang University School of Medicine, Lishui, China. 379095641@qq.com.
  • 8 Department of Orthopaedic Surgery, Lishui Central Hospital and Fifth Affiliated Hospital of Wenzhou Medical College, 289 Kuocang Road, Lishui, 323000, Zhejiang, China. 379095641@qq.com.
PMID: 40500381 DOI: 10.1007/s00210-025-04353-y
Abstract

Knee osteoarthritis (OA) has emerged as a growing public health concern in recent years. We aimed to investigate the impact of Chicago Sky Blue 6B (CSB) on OA progression in rats. In this study, we established a rat OA model using DMM surgery and conducted histological staining to assess the overall morphology of rat knee joints, as well as the content of proteoglycans and Collagen upon CSB treatment. Additionally, in vitro experiments were performed using qPCR and WB to detect the expression of matrix molecules and matrix-degrading Enzymes. The expression of relevant proteins in the MAPK/NF-kB pathway was also examined. Our in vitro experiments show that IL-1β decreased Aggrecan and COL2A1 gene expression and increased MMP3, MMP13 and ADAMTS5 gene expression. CSB treatment alleviated the IL-1β-induced inflammatory response. Further experiments indicated that CSB inhibited downstream inflammatory responses by suppressing the MAPK/NF-kB pathway. In vivo experiments confirmed the therapeutic effect of CSB in alleviating OA induced by DMM surgery in rats. We demonstrated that CSB could alleviate OA in rats through the MAPK/NF-kB pathway, highlighting its potential as a therapeutic agent.

Keywords

Anti-inflammatory; Chicago Sky Blue 6B; DMM model; Macrophage migration inhibitory factor; NF-kB pathway; Osteoarthritis.

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