2. Academic Validation
  3. Deciphering meiotic chromatin organization by SYCP3

Deciphering meiotic chromatin organization by SYCP3

  • Nucleic Acids Res. 2025 Jun 6;53(11):gkaf460. doi: 10.1093/nar/gkaf460.
Shimeng Guo 1 2,  Yiran Zhang 1,  Caifeng Fei 1,  Xiaozhao Liu 3 4 5,  Wei Xia 1,  Mengcheng Luo 6,  Gonghong Wei 7,  Weibing Qin 8,  Chengliang Xiong 1,  Honggang Li 1,  Ying Yin 3 4 5,  Ximiao He 3 4 5,  Li-Quan Zhou 1
Affiliations

Affiliations

  • 1 Institute of Reproductive Health & School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
  • 2 Chongqing Key Laboratory of Human Embryo Engineering and Precision Medicine, Center for Reproductive Medicine, Women and Children's Hospital of Chongqing Medical University, Chongqing 400016, China.
  • 3 Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
  • 4 Center for Genomics and Proteomics Research, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
  • 5 Hubei Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
  • 6 Department of Tissue and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430072, China.
  • 7 Fudan University Shanghai Cancer Center & MOE Key Laboratory of Metabolism and Molecular Medicine and Department of Biochemistry and Molecular Biology of School of Basic Medical Sciences, Shanghai Medical College of Fudan University, Shanghai 200032, China.
  • 8 NHC Key Laboratory of Male Reproduction and Genetics, Guangdong Provincial Reproductive Science Institute, Guangzhou 510600, China.
PMID: 40488283 DOI: 10.1093/nar/gkaf460
Abstract

Chromatin structure during meiosis is different from somatic cells due to the assembly of the synaptonemal complex between homologous chromosome axes. However, genome-wide organizing principles of this meiosis-specific multiprotein complex remain mysterious despite intensive super-resolution imaging analysis. Here, we profiled chromatin occupancy of SYCP3, the key chromatin organizer of synaptonemal complex, in mouse spermatocytes, and showed its enrichment at open chromatin regions. Moreover, SYCP3 occupancy was largely inherited from the leptotene to pachytene stage, facilitated by transcription and fibrous assembly, and was enriched at specific SINE repeats. We also identified SYCP1-occupied regions mainly as a subpopulation of SYCP3-occupied regions with high cohesin enrichment. Collectively, our results demonstrate genome-wide profiling of SYCP3 in mouse meiosis and reveal that its occupancy is a dynamic process modulated by chromatin-related events.

Figures
Products