2. Academic Validation
  3. Nrf2 Alleviates Colistin-Induced Nephrotoxicity by Suppressing Ferroptosis via GPX4-Mediated Lipid Peroxidation and Mitochondrial Protection

Nrf2 Alleviates Colistin-Induced Nephrotoxicity by Suppressing Ferroptosis via GPX4-Mediated Lipid Peroxidation and Mitochondrial Protection

  • J Agric Food Chem. 2025 Jun 18;73(24):15281-15295. doi: 10.1021/acs.jafc.4c13030.
Zhisheng Hu 1 2 3,  Weihua Hao 1 2 3,  Wenqing Dai 1 2 3,  Xin Gao 1 2 3,  Na Cui 1 2 3,  Minge Wang 1 2 3 4,  Liangxing Fang 1 2 3,  Jian Sun 1 2 3,  Hongyan Zhang 1 2 3,  Xiaoping Liao 1 2 3
Affiliations

Affiliations

  • 1 Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
  • 2 Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China.
  • 3 National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China.
  • 4 College of Agriculture and Biology, Liaocheng University, Liaocheng, Shandong 252000, China.
PMID: 40474460 DOI: 10.1021/acs.jafc.4c13030
Abstract

Colistin (CS)-induced nephrotoxicity remains a major clinical challenge, with its mechanisms not fully understood. This study aimed to investigate the involvement of Ferroptosis and the nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant pathway in CS-induced kidney damage. In vivo, rats treated with CS exhibited kidney injury, marked by elevated serum blood urea nitrogen (BUN) and creatinine levels, increased biomarkers (kidney injury molecule-1 (Kim-1) and neutrophil gelatinase-associated lipocalin (NGAL)), and histopathological evidence of tubular damage. Oxidative stress was observed, characterized by reduced antioxidant enzyme activities (catalase (CAT), superoxide dismutase (SOD), and glutathione (GSH)) and increased malondialdehyde (MDA) levels. Additionally, CS activated the Nrf2 pathway in renal tissues. Ferroptosis markers, including iron deposition, mitochondrial damage, and altered expression of ferroptosis-related proteins (Acyl-CoA synthetase long-chain family member 4 (ACSL4), ferritin heavy chain 1 (FTH1), and Glutathione Peroxidase 4 (GPX4)), were observed. In vitro studies with NRK-52E cells confirmed these findings, including mitochondrial dysfunction and dynamics disruption (favoring fission). Ferrostatin-1 alleviated cytotoxicity, while Nrf2 knockdown exacerbated oxidative stress, mitochondrial dysfunction, and Ferroptosis. These findings reveal that Nrf2 alleviates colistin-induced nephrotoxicity by suppressing Ferroptosis through the GPX4-mediated lipid peroxidation inhibition and mitochondrial protection, highlighting the targeted activation of the Nrf2-GPX4 axis as a promising therapeutic strategy to mitigate renal damage.

Keywords

Nrf2; colistin; ferroptosis; mitochondrial dysfunction; nephrotoxicity.

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