2. Academic Validation
  3. EIPA, A macropinocytosis blocking agent, inhibits Siniperca chuatsi rhabdovirus infection in vitro and in vivo

EIPA, A macropinocytosis blocking agent, inhibits Siniperca chuatsi rhabdovirus infection in vitro and in vivo

  • Microb Pathog. 2025 Sep:206:107751. doi: 10.1016/j.micpath.2025.107751.
Hongru Liang 1,  Xia Luo 1,  Qiang Lin 1,  Xiaozhe Fu 1,  Yinjie Niu 1,  Baofu Ma 1,  Ningqiu Li 2
Affiliations

Affiliations

  • 1 Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key, Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Guangzhou, 510380, China.
  • 2 Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key, Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Guangzhou, 510380, China. Electronic address: liningq@126.com.
PMID: 40436219 DOI: 10.1016/j.micpath.2025.107751
Abstract

Siniperca chuatsi rhabdovirus (SCRV) is a highly pathogenic virus that infects Siniperca chuatsi and causes cumulative mortality rates exceeding 90 % in fish fry. Until now, there have been no specific treatments for this virus. In this study, four different chemical inhibitors were evaluated for their ability to inhibit SCRV. Only ethyl-isopropyl amiloride (EIPA), a macropinocytosis inhibitor, effectively inhibited SCRV replication in CPB cells without cytotoxicity. Further studies indicated that EIPA inhibited an early post-entry step of viral Infection. To evaluate the effect in vivo, largemouth bass (Micropterus salmoides) were infected with SCRV and fed with EIPA. The results showed that EIPA effectively inhibited SCRV Infection in vitro and in vivo, suggesting its potential applicability as a therapeutic agent for SCRV treatment.

Keywords

EIPA; Endocytosis; Inhibit; SCRV.

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