2. Academic Validation
  3. Anaphase promoting complex subunit 10 is a potential diagnostic and prognostic biomarker in oral squamous cell carcinoma

Anaphase promoting complex subunit 10 is a potential diagnostic and prognostic biomarker in oral squamous cell carcinoma

  • Arch Oral Biol. 2025 Aug:176:106296. doi: 10.1016/j.archoralbio.2025.106296.
Lu Jia 1,  Haoyong Ning 2,  Ying Liu 3,  Pan Yang 4,  Xiaowei Cheng 5,  Bin Wang 6
Affiliations

Affiliations

  • 1 Laboratory of Tissue Regeneration and Immunology, Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing 100070, China. Electronic address: jiasandy@163.com.
  • 2 Department of Pathology, Hainan Hospital of Chinese PLA General Hospital, Sanya 572013, China. Electronic address: ninghaoyong73@163.com.
  • 3 Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing 100070, China. Electronic address: mayliu18@aliyun.com.
  • 4 Department of Oral and Maxillofacial Radiology, School of Stomatology, Capital Medical University, Beijing 100070, China. Electronic address: yangpan27@yeah.net.
  • 5 Department of Nephrology, Hainan Hospital of Chinese PLA General Hospital, The Hainan Academician Team Innovation Center, Sanya 572013, China. Electronic address: 164684840@qq.com.
  • 6 Department of Nephrology, Hainan Hospital of Chinese PLA General Hospital, The Hainan Academician Team Innovation Center, Sanya 572013, China; Senior Department of Nephrology, the First Medical Center of Chinese PLA General Hospital, National Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Medical Devices and Integrated Traditional Chinese and Western Drug Development for Severe Kidney Diseases, Beijing Key Laboratory of Digital Intelligent TCM for the Prevention and Treatment of Pan-vascular Diseases, Key Disciplines of National Administration of Traditional Chinese Medicine, Beijing 100853, China. Electronic address: binwang2000@aliyun.com.
PMID: 40408783 DOI: 10.1016/j.archoralbio.2025.106296
Abstract

Objective: To explore the role of anaphase promoting complex subunit 10 (ANAPC10) in both the diagnosis and prognosis of oral squamous cell carcinoma (OSCC).

Design: ANAPC10 expressions in OSCC tissues and adjacent normal tissues were analysed using TCGA and GEO databases. Its clinical prognostic significance was evaluated using the GEPIA tool. Signalling pathways associated with ANAPC10 were identified through GO, KEGG, and GSEA. Promoter methylation levels of ANAPC10 were assessed using the UALCAN tool. The correlation between ANAPC10 expression and tumour-infiltrating immune cells was analysed using the TIMER database. ANAPC10's role in OSCC cells was validated via CCK-8 assays, wound healing assays, cell migration assays, Apoptosis assays, and cell cycle analysis.

Results: ANAPC10 expression was significantly elevated in OSCC tissues. Increased ANAPC10 expression was associated with advanced T stages, pathological stages, histologic grades, and poorer therapeutic outcomes. Notably, high ANAPC10 expression was strongly correlated with reduced overall survival, disease-specific survival, and progression-free interval in OSCC patients. Functional enrichment analyses revealed that ANAPC10 is involved in RNA splicing, immune regulation, and cell cycle progression. Experimental validation further demonstrated that ANAPC10 levels are influenced by promoter methylation status, and ANAPC10 regulates oral Cancer cell proliferation, migration, Apoptosis, and cell cycle progression.

Conclusion: ANAPC10 is a critical gene in OSCC prognosis, with roles in cell cycle regulation and RNA splicing. It may serve as a diagnostic biomarker and therapeutic target.

Keywords

Anaphase promoting complex subunit 10; Cell cycle; Immune infiltration; Oral squamous cell carcinoma; RNA splicing.

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