2. Academic Validation
  3. 5,7,2',6'- Tetrahydroxyflavone affects the progression of ovarian cancer via hsa-miR-495-3p-ACTB/HSP90AA1 pathway

5,7,2',6'- Tetrahydroxyflavone affects the progression of ovarian cancer via hsa-miR-495-3p-ACTB/HSP90AA1 pathway

  • Discov Oncol. 2025 May 19;16(1):817. doi: 10.1007/s12672-025-02570-8.
Mengjie Chen # 1 2,  Huihui Jia # 3,  Xuyang Tao 4,  Yani Jin 2,  Zuorong Shi 5
Affiliations

Affiliations

  • 1 School of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.
  • 2 Department of Gynecology, Xi 'an Hospital of Traditional Chinese Medicine, Xi 'an, 710000, China.
  • 3 Department of Gynecology and Obstetrics, Xi 'an No.1 Hospital (High-tech campus), Xi 'an, 710100, China.
  • 4 Traditional Chinese Medicine Department, Xi 'an No.1 Hospital (High-Tech Campus), Xi 'an, 710100, China.
  • 5 Admission Office, Shandong University of Traditional Chinese Medicine, No. 4655 University Road, Science and Technology Park, Changqing District, Jinan, 250355, Shandong, China. zuorongshi963@163.com.
  • # Contributed equally.
PMID: 40389695 DOI: 10.1007/s12672-025-02570-8
Abstract

Background: Scutellariae Radix (SR), a traditional Chinese medicine, has been shown to have potential anti-cancer properties.

Purpose: To explore the mechanism of inhibiting ovarian Cancer (OC) progression by SR.

Methods: The key active ingredient (5,7,2',6'-Tetrahydroxyflavone, TF) and key targets (ACTB and HSP90AA1) of SR were screened by the network pharmacology method. CCK-8 reagent, Transwell assay, and Annexin-V-FITC kit were used to evaluate the effects of TF on OC cell viability, migration, and Apoptosis. The upstream MicroRNAs (miRNAs) of ACTB and HSP90AA1 were predicted by the starBase database. Important miRNAs related to OC were mined using gene expression datasets in the GEO database. RT-qPCR and Western blotting experiments were used to detect miRNA or gene expression.

Results: TF inhibited OC cell viability/migration and induced Apoptosis in a concentration-dependent manner. Hsa-miR-495-3p was identified to be a key miRNA in OC, whose expression was lacking in OC cells. ACTB and HSP90AA1 expressed highly in OC cells. Hsa-miR-495-3p mimics reduced ACTB and HSP90AA1 expression. Hsa-miR-495-3p inhibitor and overexpression of ACTB or HSP90AA1 reversed the inhibitory effect of TF on OC cells.

Conclusion: TF, an active ingredient of SR, hindered OC progression through the hsa-miR-495-3p-ACTB/HSP90AA1 pathway.

Keywords

Scutellariae Radix; ACTB; HSP90AA1; Hsa-miR-495-3p; Ovarian cancer.

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