2. Academic Validation
  3. Puerarin Alleviates Alcoholic Liver Disease via Suppressing Lipolysis Induced by Sympathetic Outflow

Puerarin Alleviates Alcoholic Liver Disease via Suppressing Lipolysis Induced by Sympathetic Outflow

  • Am J Chin Med. 2025;53(3):863-888. doi: 10.1142/S0192415X25500326.
Ke Zheng 1,  Liu Yang 1,  Rui-Shuo Zhang 2,  Yi-Han Qian 1,  Yu-Ge Zhou 1,  Wei-Fan Huang 1,  Jia-Cheng Lin 1,  Yan-Jun Shi 3,  Xiao-Ni Kong 1
Affiliations

Affiliations

  • 1 Central Laboratory, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
  • 2 Clinical Medicine Admitted Class of 2020, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230031, China.
  • 3 Abdominal Transplantation Center, General Surgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China.
PMID: 40374377 DOI: 10.1142/S0192415X25500326
Abstract

The aim of this study was to evaluate the therapeutic effect of puerarin (PUE) on alcoholic liver disease (ALD) and elucidate the potential mechanism from the perspective of lipolysis and hepatic steatosis. Assessment of PUE efficacy against ALD was performed using serum biochemical parameters and the histological examination of liver and adipose tissue via Hematoxylin and eosin (H&E) staining. The potential mechanisms underlying the amelioration of ALD by PUE were investigated using Western blotting (WB) analysis and immunofluorescence (IHC) staining. We demonstrated that PUE attenuated steatosis in ALD by alleviating ethanol-induced liver damage and lipid accumulation, suppressing the expression of lipid synthesis genes, upregulating the expression of lipid metabolism genes, and reducing lipolysis by inhibiting adipose triglyceride Lipase (ATGL) activation and the phosphorylation of hormone-sensitive Lipase (HSL). In conclusion, PUE ameliorates ALD by inhibiting the sympathetic outflow-mediated activation of key lipolysis Enzymes ATGL and HSL. These findings provide a solid theoretical foundation for the potential application of PUE in the clinical treatment of ALD.

Keywords

Alcoholic Liver Disease; Lipolysis; Puerarin; Sympathetic Outflow.

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