2. Academic Validation
  3. Sunitinib-resistant renal cell carcinoma cell-derived exosomes promote facilitation of tumor progression via secretion of the lncRNA SNHG16

Sunitinib-resistant renal cell carcinoma cell-derived exosomes promote facilitation of tumor progression via secretion of the lncRNA SNHG16

  • Hum Cell. 2025 May 13;38(4):100. doi: 10.1007/s13577-025-01228-5.
WeiLijiang Saimaiti 1,  Jun Ma 2,  Paluoke Dilimulati 1,  Yujie Wang 3
Affiliations

Affiliations

  • 1 Department of Pediatric Urology, First Affiliated Hospital, Xinjiang Medical University, Urumqi, 830054, China.
  • 2 Department of Urology, First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyuushan South Road, Urumqi, 830054, Xinjiang, China.
  • 3 Department of Urology, First Affiliated Hospital, Xinjiang Medical University, No. 137, Liyuushan South Road, Urumqi, 830054, Xinjiang, China. 13699999610@139.com.
PMID: 40358820 DOI: 10.1007/s13577-025-01228-5
Abstract

Renal cell carcinoma (RCC) is one of the most common tumors of high malignancy in the urological system. Sunitinib is commonly used to treat RCC, while drug resistance severely limited the therapeutic efficacy. Tumor-derived exosomes play important roles in facilitating Cancer development. However, the role of drug-resistant tumor-derived exosomes in tumorigenesis and resistance of RCC has not been elucidated. Here we isolated sunitinib-sensitive/resistant RCC cells-derived exosomes, characterized by transmission electron microscopy (TEM) and western blot. Furthermore, co-culture experiments were performed and we found that sunitinib-resistant RCC cells-derived exosomes (R-exos) promoted cell proliferation and upregulated proliferation-related genes cyclin D1 (CCND1) and proliferating cell nuclear antigen (PCNA) expression, and inhibited Apoptosis and the expression of Bax and Caspase-3 of sunitinib-resistant RCC (RCC/R) cells by delivering lncRNA small nuclear RNA host gene 16 (SNHG16). In resistant cell-derived xenograft (CDX-R) models, R-exos induced tumor growth in vivo, while knockdown of SNHG16 effectively diminished the tumorigenesis of RCC. Moreover, SNHG16 positively regulated the expression of trophinin associated protein (TROAP) by sponging miR-106a-5p in RCC cells, whereas inhibition of miR-106a-5p or overexpression of TROAP greatly reversed the suppression of tumorigenesis and sunitinib resistant by silencing SNHG16. R-exos lncRNA SNHG16 promoted sunitinib resistant and malignant progress by regulating the miR-106a-5p/TROAP axis, and targeting SNHG16/miR-106a-5p/TROAP axis may be a novel therapeutic approach for sunitinib-treated patients of RCC.

Keywords

LncRNA; Renal cell carcinoma; Resistant; Sunitinib; Tumor-derived exosomes.

Figures
Products