2. Academic Validation
  3. Forskolin-loaded carboxymethyl chitosan and silk nonofiber hydrogels composite scaffolds improve skin regeneration

Forskolin-loaded carboxymethyl chitosan and silk nonofiber hydrogels composite scaffolds improve skin regeneration

  • Int J Biol Macromol. 2025 May;310(Pt 1):142931. doi: 10.1016/j.ijbiomac.2025.142931.
Lingzhi Zhong 1,  Cuijuan Shi 2,  Meirong Li 3,  Qian Hou 4,  Jifang Yuan 4,  Chengyong Xu 5,  Dandan Song 1,  Tao He 1,  Fangrui Zhu 1,  Peifeng Li 6,  Yuzhen Li 7
Affiliations

Affiliations

  • 1 Basic Medical Department, Graduate School, PLA General Hospital and PLA Medical College, Beijing 100853, China.
  • 2 Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China.
  • 3 Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department, PLA General Hospital and PLA Medical College, Beijing 100853, China; Center for Drug Evaluation, National Medical Products Administration, Beijing 100076, China.
  • 4 Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department, PLA General Hospital and PLA Medical College, Beijing 100853, China.
  • 5 Rehabilitation Department, Hainan Hospital of PLA General Hospital, Sanya 572013, Hainan, China.
  • 6 Institute of Life and Health, Zhengzhou University of Light Industry, Zhengzhou 450001, Henan, China. Electronic address: peifengli@zzuli.edu.cn.
  • 7 Basic Medical Department, Graduate School, PLA General Hospital and PLA Medical College, Beijing 100853, China. Electronic address: yuzlif96@163.com.
PMID: 40239781 DOI: 10.1016/j.ijbiomac.2025.142931
Abstract

Hydrogel is a kind of carrier of powerful therapeutic bioactive molecules. Here, carboxymethyl chitosan-silk nanofiber (CCSF) composite hydrogels loaded with a small molecule compound, Forskolin, were prepared to achieve sustained release of the small molecule drug for promoting wound healing. In vitro, investigations confirmed that the innovative composite dressing is non-toxic and cyto-compatible. Specifically, it can encourage the proliferation of human umbilical vein endothelial cells (HUVECs) and mouse dermis fibroblasts, and inhibit the accumulation of intracellular Reactive Oxygen Species (ROS) in the cells. The capacity of cell migration in myofibroblasts was restrained, also implying less scarring in vivo. Skin wound regeneration results showed that the Forskolin-loaded compound hydrogel accelerated wound closure, enhanced neovascularization, and promoted hair follicle regeneration. The multiple-material delivery system shows a promising application in skin wound repair.

Keywords

Carboxymethyl chitosan; Hydrogel; Nanofiber; Skin regeneration; Sustained release.

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