2. Academic Validation
  3. Intraarterial Infusion of Lidocaine is Superior to the Subcutaneous Injection of Low Molecular Weight Heparin for Improving the Course of Cerulein-Induced Acute Pancreatitis in Rats

Intraarterial Infusion of Lidocaine is Superior to the Subcutaneous Injection of Low Molecular Weight Heparin for Improving the Course of Cerulein-Induced Acute Pancreatitis in Rats

  • Arch Immunol Ther Exp (Warsz). 2025 Apr 16;73(1). doi: 10.2478/aite-2025-0012.
Ryszard Antkowiak 1,  Lukasz Antkowiak 2 3,  Zbigniew Arent 4,  Bogna Drozdzowska 5,  Anna Kasperczuk 6,  Jacek Bialecki 7,  Agnieszka Pietsch-Fulbiszewska 4,  Pawel Domoslawski 8,  Agata Cieslik-Bielecka 9,  Marek Kucharzewski 10
Affiliations

Affiliations

  • 1 Department of General and Oncologic Surgery, St. Joseph Hospital in Mikolow, Mikolow, Poland.
  • 2 Department of Pediatric Neurosurgery, Medical University of Silesia in Katowice, Katowice, Poland.
  • 3 Department of Neurosurgery, St. Barbara Regional Hospital and Trauma Center in Sosnowiec, Medical University of Silesia in Katowice, Sosnowiec, Poland.
  • 4 Experimental and Innovative Medicine Centre, University of Agriculture, Krakow, Poland.
  • 5 Department of Pathomorphology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland.
  • 6 Faculty of Mechanical Engineering, Institute of Biomedical Engineering, Bialystok University of Technology, Bialystok, Poland.
  • 7 Department of General Minimally Invasive and Trauma Surgery, Francis Raszeja Municipal Hospital, Poznan, Poland.
  • 8 Department of General Gastroenterologic and Endocrine Surgery, Wroclaw Medical University, Wroclaw, Poland.
  • 9 Department of Oral and Maxillofacial Surgery, St. Barbara Regional Hospital and Trauma Center in Sosnowiec, Sosnowiec, Poland.
  • 10 Wladyslaw Bieganski Collegium Medicum, Jan Długosz University in Czestochowa, Czestochowa, Poland.
PMID: 40237149 DOI: 10.2478/aite-2025-0012
Abstract

This study aimed to determine the efficacy of low molecular weight heparin (LMWH) and lidocaine combined with LMWH for improving the course of acute pancreatitis (AP). A total of 30 rats were divided into three groups: the NaCl group, which received an intraarterial infusion of 0.9% sodium chloride; the Heparin group, which received a subcutaneous injection of LMWH; and the Lidocaine-Heparin group, which received an intraarterial infusion of 1% lidocaine, with subsequent subcutaneous injection of LMWH. AP was triggered using 80 μg/kg body weight of cerulein. Serum amylase and Lipase levels were evaluated before induction of AP (measurement 0 - M0), after triggering AP (measurement 1 - M1), 1 h (measurement 2 - M2), 3 h (measurement 3 - M3), and 5 h (measurement 4 - M4) after treatment. After euthanasia, pancreatic tissues were collected for pathological analysis. No intergroup differences in serum amylase and Lipase levels were observed between the NaCl and Heparin groups in all post-treatment evaluation points (M2, M3, and M4). Conversely, the Lidocaine-Heparin group showed significantly lower amylase values than the NaCl and Heparin groups in all post-treatment evaluation points. Furthermore, the Lidocaine-Heparin group showed significantly lower Lipase values compared with the NaCl group in the first post-treatment evaluation point (M2), as well as compared with the Heparin group in the first (M2) and second (M3) post-treatment evaluation points. No significant intergroup differences were observed in pathological pancreatic tissue evaluation. Subcutaneous injection of LMWH did not impact the natural course of AP. However, the addition of intraarterially administered 1% lidocaine solution significantly reduced the severity of AP.

Keywords

Anticoagulation; Experimental; Microcirculation; Vasodilation; continuous regional arterial infusion.

Figures
Products