2. Academic Validation
  3. Miquelianin inhibits IAV infection via the MAPK signaling pathway both in vitro and in vivo

Miquelianin inhibits IAV infection via the MAPK signaling pathway both in vitro and in vivo

  • Front Immunol. 2025 Mar 17:16:1532336. doi: 10.3389/fimmu.2025.1532336.
He Li # 1,  Beilei Shen # 2,  Yan Bi # 1,  Yan Sun 3,  Shijun Zhang 1,  Kun Xue 2,  Qiuyue Wang 4,  Bingshuo Qian 5,  Junkui Zhang 5,  Lingjun Fan 6,  Zhengyuan Fang 1,  Tiecheng Wang 2,  Yuwei Gao 2,  Donghui Yue 1
Affiliations

Affiliations

  • 1 College of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, China.
  • 2 Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China.
  • 3 College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, China.
  • 4 College of Traditional Chinese Medicine, Hainan Medical University, Haikou, Hainan, China.
  • 5 School of Pharmacy, Henan University, Kaifeng, China.
  • 6 Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, China.
  • # Contributed equally.
PMID: 40165966 DOI: 10.3389/fimmu.2025.1532336
Abstract

Background: Influenza is an acute respiratory infectious disease primarily transmitted through airborne droplets. The prevalence and spread of influenza viruses have significant impacts on global economic development and public health. Current prevention and control strategies for Influenza Virus infections mainly rely on vaccines and Antiviral drugs. However, vaccine efficacy is limited by the antigenic drift and mutation characteristics of influenza viruses, while Antiviral drug resistance is increasingly prevalent. Therefore, there is an urgent need for the development of novel Antiviral agents. Flavonoids, widely distributed in Plants, possess various potent biological properties, including antioxidant, anti-inflammatory, Antibacterial, and Anticancer activities, which contribute to the management and prevention of numerous diseases. This study aims to investigate the in vitro and in vivo anti-influenza A virus activity of quercetin, taxifolin, and miquelianin, as well as their underlying.

Methods: In vitro Infection model (MDCK cells) and mouse lethal Infection model of Infuenza A virus were used to evaluate the Antiviral activity of quercetin, taxifolin and miquelianin. Subsequently, we applied network pharmacology to elucidate the mechanism of action and validate the findings for miquelianin.

Results: Miquelianin effectively inhibits the replication of H1N1-UI182 both in vitro and in vivo and provides protection against lethal H1N1-UI182 Infection in mice. Compared to virus-infected controls, miquelianin reduces lung injury. Furthermore, by inhibiting the MAPK signaling pathway, miquelianin prevents the overproduction of cytokines, such as IL-6 and IL-1β, induced by viral Infection, thereby alleviating inflammatory responses.

Conclusion: Miquelianin is a monomer extracted from traditional Chinese medicine, exhibiting inhibitory effects on H1N1-UI182 replication and lung injury mitigation.

Keywords

MAPK signaling pathway; flavonoids; influenza A virus; miquelianin; network pharmacology.

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