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  3. Single cell RNA sequencing shows that cells expressing Sox9 postnatally populate most skeletal lineages in mouse bone

Single cell RNA sequencing shows that cells expressing Sox9 postnatally populate most skeletal lineages in mouse bone

  • J Bone Miner Res. 2025 Jun 3;40(6):799-812. doi: 10.1093/jbmr/zjaf043.
Neal P Smith 1,  Christopher Janton 2 3,  Ana Clara Morellato Alcantara 2 4,  Majd George 2,  Kasidet Mankongtreecheep 1 5,  Guping Mao 2 6,  Alexandra-Chloé Villani 1 7,  Henry M Kronenberg 2 7
Affiliations

Affiliations

  • 1 Department of Medicine, Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, MA 02114, United States.
  • 2 Endocrine Unit, Massachusetts General Hospital, Boston, MA 02114, United States.
  • 3 New York Institute of Technology College of Osteopathic Medicine, Old Westbury, NY, 11568, United States.
  • 4 West Virginia School of Medicine, Morgantown, WV 26506, United States.
  • 5 Dana Farber Cancer Center, Boston, MA 02115, United States.
  • 6 Department of Orthopaedic Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
  • 7 Department of Medicine, Harvard Medical School, Boston, MA 02115, United States.
PMID: 40143416 DOI: 10.1093/jbmr/zjaf043
Abstract

In growing bones of mice, multiple cell types contribute to the osteoblast lineage, including growth plate chondrocytes, perichondrial cells and CXCL12-abundant reticular marrow stromal cells. Here we use single-cell RNA Sequencing and lineage tracing to show that all these osteoblast precursors, even postnatally, derives from Sox9-expressing progenitors. We also characterize a distinct group of chondrocytes located between the perichondrium and the columns of growth plate chondrocytes that contribute to the osteoblast lineage.

Keywords

biology; bone modeling and remodeling; cells of bone; chondrocyte and cartilage; chondrocyte and cartilage biology; developmental modeling; growth plate; osteoblasts; stromal/stem cells.

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