2. Academic Validation
  3. miR-NPs-RVG promote spinal cord injury repair: implications from spinal cord-derived microvascular endothelial cells

miR-NPs-RVG promote spinal cord injury repair: implications from spinal cord-derived microvascular endothelial cells

  • J Nanobiotechnology. 2024 Sep 28;22(1):590. doi: 10.1186/s12951-024-02797-7.
Chao Li # 1,  Zhenyang Xiang # 1,  Mengfan Hou 1,  Hao Yu 1,  Peng Peng 1,  Yigang Lv 1,  Chao Ma 1,  Han Ding 1,  Yunpeng Jiang 2,  Yang Liu 3,  Hengxing Zhou 4 5,  Shiqing Feng 6 7 8
Affiliations

Affiliations

  • 1 Tianjin Key Laboratory of Spine and Spinal Cord, Department of Orthopaedics, International Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Medical University General Hospital, Tianjin, 300052, People's Republic of China.
  • 2 Department of Orthopaedics, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, People's Republic of China.
  • 3 Tianjin Key Laboratory of Spine and Spinal Cord, Department of Orthopaedics, International Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Medical University General Hospital, Tianjin, 300052, People's Republic of China. liuyangda666@foxmail.com.
  • 4 Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Advanced Medical Research Institute, Cheeloo College of Medicine, Shandong University, Jinan, 250012, People's Republic of China. kevin_zyf@126.com.
  • 5 Center for Reproductive Medicine, Shandong University, Jinan, 250012, Shandong, People's Republic of China. kevin_zyf@126.com.
  • 6 Tianjin Key Laboratory of Spine and Spinal Cord, Department of Orthopaedics, International Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Medical University General Hospital, Tianjin, 300052, People's Republic of China. sqfeng@tmu.edu.cn.
  • 7 Department of Orthopaedics, Qilu Hospital of Shandong University, Shandong University Centre for Orthopaedics, Advanced Medical Research Institute, Cheeloo College of Medicine, Shandong University, Jinan, 250012, People's Republic of China. sqfeng@tmu.edu.cn.
  • 8 The Second Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, 250033, Shandong, People's Republic of China. sqfeng@tmu.edu.cn.
  • # Contributed equally.
PMID: 39342236 DOI: 10.1186/s12951-024-02797-7
Abstract

Background: Spinal cord injury (SCI) often leads to a loss of motor and sensory function. Axon regeneration and outgrowth are key events for functional recovery after spinal cord injury. Endogenous growth of axons is associated with a variety of factors. Inspired by the relationship between developing nerves and blood vessels, we believe spinal cord-derived microvascular endothelial cells (SCMECs) play an important role in axon growth.

Results: We found SCMECs could promote axon growth when co-cultured with neurons in direct and indirect co-culture systems via downregulating the miR-323-5p expression of neurons. In rats with spinal cord injury, neuron-targeting nanoparticles were employed to regulate miR-323-5p expression in residual neurons and promote function recovery.

Conclusions: Our study suggests that SCMEC can promote axon outgrowth by downregulating miR-323-5p expression within neurons, and miR-323-5p could be selected as a potential target for spinal cord injury repair.

Keywords

Nanoparticles; Spinal cord injury; Spinal cord-derived microvascular endothelial cells; miR-323-5p.

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