2. Academic Validation
  3. Laurocapram, a transdermal enhancer, boosts cephalosporin's antibacterial activity against Methicillin-resistant Staphylococcus aureus

Laurocapram, a transdermal enhancer, boosts cephalosporin's antibacterial activity against Methicillin-resistant Staphylococcus aureus

  • Biochem Pharmacol. 2024 Sep:227:116404. doi: 10.1016/j.bcp.2024.116404.
Nan Wang 1,  Wei Li 2,  Hui Yu 3,  Wenlin Huang 3,  Yarui Qiao 4,  Qi Wang 5,  Yunfei Wei 3,  Xuming Deng 1,  Jianfeng Wang 3,  Minhe Cui 6,  Peng Zhang 7,  Yonglin Zhou 8
Affiliations

Affiliations

  • 1 Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun 130021, China; State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130062, China.
  • 2 Department of Gastrocolorectal Surgery, General Surgery Center, The First Hospital of Jilin University, 71 Xinmin Street, Changchun 130021, Jilin, China.
  • 3 State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130062, China.
  • 4 Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, and School of Life Sciences, Ningxia University, Yinchuan, China.
  • 5 College of Life Science, Jilin Agricultural University, 130118 Changchun, China.
  • 6 Jilin Mushuo Breeding Co., Ltd, Changchun 130052, Jilin, China.
  • 7 Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun 130021, China. Electronic address: zhangpengjdyy@jlu.edu.cn.
  • 8 Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China, and School of Life Sciences, Ningxia University, Yinchuan, China. Electronic address: Zhouyonglin333@jlu.edu.cn.
PMID: 38945279 DOI: 10.1016/j.bcp.2024.116404
Abstract

Methicillin-resistant Staphylococcus aureus (MRSA), a notorious bacterium with high drug resistance and easy recurrence after surgery, has posed significant clinical treatment challenges. In the current scarcity of new Antibiotics, the identification of adjuvants to existing Antibiotics is a promising approach to combat infections caused by multidrug-resistant Gram-positive bacteria. The in vitro synergy test, which included a MIC assay, time-kill curve, antimicrobial susceptibility testing, and live/dead bacteria staining assay, revealed that laurocapram, a widely used chemical transdermal enhancer, could potentiate the Antibacterial activity of cephalosporins against MRSA. In vitro, laurocapram combined with cefixime showed an excellent synergistic activity against MRSA (FICI = 0.28 ± 0.00). In addition, the combination of laurocapram and cefixime may inhibited the formation of MRSA biofilm and caused cell membrane damage. Following that, we discovered that combining laurocapram with cefixime could alleviate the symptoms of mice in the MRSA skin Infection model and the MRSA pneumonia model. In conclusion, laurocapram is a promising and low-cost Antibacterial adjuvant, providing a new strategy for further exploring the use of lower doses of cephalosporins to combat MRSA Infection.

Keywords

Cefixime; Laurocapram; Methicillin-resistant Staphylococcus aureus; Synergistic activity.

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