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  3. Solubilization of fish myofibrillar proteins in NaCl and KCl solutions: A DIA-based proteomics analysis

Solubilization of fish myofibrillar proteins in NaCl and KCl solutions: A DIA-based proteomics analysis

  • Food Chem. 2024 Jul 1:445:138662. doi: 10.1016/j.foodchem.2024.138662.
Xidong Jiao 1,  Xingying Li 2,  Nana Zhang 3,  Bowen Yan 1,  Jianlian Huang 4,  Jianxin Zhao 2,  Hao Zhang 2,  Wei Chen 2,  Daming Fan 5
Affiliations

Affiliations

  • 1 State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing, Ministry of Agriculture and Rural Affairs, Xiamen 361022, China.
  • 2 State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
  • 3 State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing, Ministry of Agriculture and Rural Affairs, Xiamen 361022, China. Electronic address: nanazhang@jiangnan.edu.cn.
  • 4 ANJOY FOODS GROUP CO., LTD., Xiamen 361022, China; Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing, Ministry of Agriculture and Rural Affairs, Xiamen 361022, China; Engineering Technology Research Center of Refrigeration and Conditioning Aquatic Food (Liaoning Anjoy Food Co., LTD), China National Light Industry Council, Anshan 114100, China.
  • 5 State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Refrigeration and Conditioning Aquatic Products Processing, Ministry of Agriculture and Rural Affairs, Xiamen 361022, China; Engineering Technology Research Center of Refrigeration and Conditioning Aquatic Food (Liaoning Anjoy Food Co., LTD), China National Light Industry Council, Anshan 114100, China. Electronic address: fandm@jiangnan.edu.cn.
PMID: 38354641 DOI: 10.1016/j.foodchem.2024.138662
Abstract

Understanding the basic solubilization of fish myofibrillar proteins (MPs) in common monovalent chloride solutions is crucial for muscle food processing. In this study, the differential proteomic profiles of MPs during extraction and solubilization in NaCl and KCl solutions were investigated by using advanced four-dimensional data-independent acquisition (4D DIA) quantitative proteomics for the first time. Compared to routine biochemical analysis, this could provide insights into the solubilization of muscle proteins. We ensure the consistency of the effective ionic strength of NaCl and KCl buffers by adjusting the conductivity. The results showed that NaCl extractor mainly facilitated the solubilization of cytoskeletal proteins, biochemical Enzymes, and stromal proteins compared to KCl, such as tubulin, myosin-9, Collagen, plectin, protein Phosphatase, and Cathepsin D. However, no significant difference was observed in the extraction of major sarcomeric proteins, including Myosin, actin, troponin C, myosin-binding protein C, M-Protein, α-actinin-3, and tropomyosin.

Keywords

4D DIA proteomics; Fish; KCl; Myofibrillar proteins; NaCl; Solubilization.

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