2. Academic Validation
  3. Uncovering the anti-biofilm activity of Ilicicolin B against Staphylococcus aureus

Uncovering the anti-biofilm activity of Ilicicolin B against Staphylococcus aureus

  • Biochem Biophys Res Commun. 2023 Dec 3:684:149138. doi: 10.1016/j.bbrc.2023.149138.
Ziyi Tang 1,  Jizhou Feng 2,  Sankara Rao Rowthu 2,  Cheng Zou 2,  Haibo Peng 3,  Chao Huang 2,  Yun He 4
Affiliations

Affiliations

  • 1 Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China.
  • 2 Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China.
  • 3 Chongqing Academy of Science and Technology, Chongqing, 401123, China.
  • 4 Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing, 401331, China; BayRay Innovation Center, Shenzhen Bay Laboratory, Shenzhen, 518132, China. Electronic address: yun.he@cqu.edu.cn.
PMID: 37897909 DOI: 10.1016/j.bbrc.2023.149138
Abstract

The formation of Bacterial biofilms reduces the entry of Antibiotics into bacteria and helps bacteria tolerate otherwise lethal concentrations of antimicrobials, leading to Antibiotic resistance. Therefore, clearing Bacterial biofilm is an effective strategy to tackle drug resistance. Currently, there are no approved Antibiotics for inhibiting Bacterial biofilm formation. We found that Ilicicolin B had excellent Antibacterial activity against MRSA without obvious hemolytic activity. More importantly, Ilicicolin B effectively inhibited the biofilm formation in a concentration-dependent manner by crystal violet colorimetric assay and fluorescence microscopy analysis. Exposure of Staphylococcus aureus to Ilicicolin B for 24 h reduced the protein and polysaccharide components in EPS, suggesting that Ilicicolin B disintegrated the biofilms by dissociating the EPS in a matrix. In addition, Ilicicolin B demonstrated strong Antibacterial effects in a murine abscess model of S. aureus. Our findings suggest that Ilicicolin B has the potential to treat S. aureus Infection by inhibiting biofilm formation.

Keywords

Anti-biofilm activity; Antibacterial; Extracellular polymeric substances (EPS); Ilicicolin B; Staphylococcus aureus.

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