2. Academic Validation
  3. Integrated Omic Analysis of Human Plasma Metabolites and Microbiota in a Hypertension Cohort

Integrated Omic Analysis of Human Plasma Metabolites and Microbiota in a Hypertension Cohort

  • Nutrients. 2023 Apr 25;15(9):2074. doi: 10.3390/nu15092074.
Bo-Yan Chen 1 2 3,  Yu-Lin Li 2 3,  Wen-Zhen Lin 2 3,  Chao Bi 4,  Lin-Juan Du 2 3,  Yuan Liu 2 3,  Lu-Jun Zhou 2 3 5,  Ting Liu 2 3,  Shuo Xu 2 3,  Jun Zhang 2 3,  Yan Liu 2 3,  Hong Zhu 2 3,  Wu-Chang Zhang 2 3,  Zhi-Yuan Zhang 1 3,  Sheng-Zhong Duan 2 3
Affiliations

Affiliations

  • 1 Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
  • 2 Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China.
  • 3 National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai 200125, China.
  • 4 Department of Stomatology, First Affiliated Hospital, Anhui Medical University, Hefei 230022, China.
  • 5 Department of General Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
PMID: 37432207 DOI: 10.3390/nu15092074
Abstract

Hypertension is closely related to metabolic dysregulation, which is associated with microbial dysbiosis and altered host-microbiota interactions. However, plasma metabolite profiles and their relationships to oral/gut microbiota in hypertension have not been evaluated in depth. Plasma, saliva, subgingival plaques, and feces were collected from 52 hypertensive participants and 24 healthy controls in a cross-sectional cohort. Untargeted metabolomic profiling of plasma was performed using high-performance liquid chromatography-mass spectrometry. Microbial profiling of oral and gut samples was determined via 16S rRNA and metagenomic Sequencing. Correlations between metabolites and clinic parameters/microbiota were identified using Spearman's correlation analysis. Metabolomic evaluation showed distinct clusters of metabolites in plasma between hypertensive participants and control participants. Hypertensive participants had six significantly increased and thirty-seven significantly decreased plasma metabolites compared to controls. The plasma metabolic similarity significantly correlated with the community similarity of microbiota. Both oral and gut microbial community composition had significant correlations with metabolites such as Sphingosine 1-phosphate, a molecule involved in the regulation of blood pressure. Plasma metabolites had a larger number of significant correlations with Bacterial genera than Fungal genera. The shared oral/gut Bacterial genera had more correlations with metabolites than unique genera but shared Fungal genera and metabolites did not show clear clusters. The hypertension group had fewer correlations between plasma metabolites and bacteria/fungi than controls at species level. The integrative analysis of plasma metabolome and oral/gut microbiome identified unreported alterations of plasma metabolites in hypertension and revealed correlations between altered metabolites and oral/gut microbiota. These observations suggested metabolites and microbiota may become valuable targets for therapeutic and preventive interventions of hypertension.

Keywords

gut microbiota; hypertension; oral microbiota; plasma metabolites.

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