2. Academic Validation
  3. Antimicrobial Natural Products from Plant Pathogenic Fungi

Antimicrobial Natural Products from Plant Pathogenic Fungi

  • Molecules. 2023 Jan 23;28(3):1142. doi: 10.3390/molecules28031142.
Melissa M Cadelis 1 2,  Steven A Li 1,  Shara J van de Pas 2,  Alex Grey 2,  Daniel Mulholland 2,  Bevan S Weir 3,  Brent R Copp 1,  Siouxsie Wiles 2 4
Affiliations

Affiliations

  • 1 School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
  • 2 Bioluminescent Superbugs Lab, School of Medical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
  • 3 Manaaki Whenua-Landcare Research, Private Bag 92170, Auckland 1142, New Zealand.
  • 4 Te Pūnaha Matatini Centre of Research Excellence in Complex Systems, Auckland 1142, New Zealand.
PMID: 36770808 DOI: 10.3390/molecules28031142
Abstract

Isolates of a variety of Fungal plant pathogens (Alternaria radicina ICMP 5619, Cercospora beticola ICMP 15907, Dactylonectria macrodidyma ICMP 16789, D. torresensis ICMP 20542, Ilyonectria europaea ICMP 16794, and I. liriodendra ICMP 16795) were screened for antimicrobial activity against the human pathogenic bacteria Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Mycobacterium abscessus, and M. marinum and were found to have some activity. Investigation of the secondary metabolites of these Fungal isolates led to the isolation of ten natural products (1-10) of which one was novel, (E)-4,7-dihydroxyoct-2-enoic acid (1). Structure elucidation of all natural products was achieved by a combination of NMR spectroscopy and mass spectrometry. We also investigated the antimicrobial activity of a number of the isolated natural products. While we did not find (E)-4,7-dihydroxyoct-2-enoic acid (1) to have any activity against the bacteria and fungi in our assays, we did find that cercosporin (7) exhibited potent activity against Methicillin resistant Staphylococcus aureus (MRSA), dehydro-curvularin (6) and radicicol (10) exhibited antimycobacterial activity against M. marinum, and brefeldin A (8) and radicicol (10) exhibited Antifungal activity against Candida albicans. Investigation of the cytotoxicity and haemolytic activities of these natural products (6-8 and 10) found that only one of the four active compounds, radicicol (10), was non-cytotoxic and non-haemolytic.

Keywords

Candida albicans; Staphylocccus aureus; antibacterial activity; fungal plant pathogens; isolation; metabolites; mycobacteria; natural product.

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