2. Academic Validation
  3. A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging

A visualization pipeline for in vivo two-photon volumetric astrocytic calcium imaging

  • J Biomed Res. 2022 Aug 10;36(5):358-367. doi: 10.7555/JBR.36.20220099.
Qian Sun 1,  Yusi Hu 1 2,  Saiyue Deng 3,  Yanyu Xiong 1 2,  Zhili Huang 1 2
Affiliations

Affiliations

  • 1 Department of Pharmacology, School of Basic Medical Science, Fudan University, Shanghai 200032, China.
  • 2 State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China.
  • 3 Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
PMID: 36130733 DOI: 10.7555/JBR.36.20220099
Abstract

Astrocytes, the multi-functional glial cells with the most abundant population in the brain, integrate information across their territories to regulate neuronal synaptic and cerebrovascular activities. Astrocytic calcium (CA 2+) signaling is the major readout of cellular functional state of astrocytes. The conventional two-photon in vivo imaging usually focuses on a single horizontal focal plane to capture the astrocytic CA 2+ signals, which leaves >80% spatial information undetected. To fully probe the CA 2+ activity across the whole astrocytic territory, we developed a pipeline for imaging and visualizing volumetric astrocytic CA 2+ time-lapse images. With the pipeline, we discovered a new signal distribution pattern from three-dimensional (3D) astrocytic CA 2+ imaging data of mice under isoflurane anesthetic states. The tools developed in this study enable a better understanding of the spatiotemporal patterns of astrocytic activity in 3D space.

Keywords

astrocyte; calcium imaging; three-dimensional visualization.

Figures
Products