2. Academic Validation
  3. Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana

  • Bio Protoc. 2018 May 20;8(10):e2844. doi: 10.21769/BioProtoc.2844.
Valérie Allasia 1,  Benoit Industri 1,  Michel Ponchet 1,  Michaël Quentin 1,  Bruno Favery 1,  Harald Keller 1
Affiliations

Affiliation

  • 1 INRA, Université Côte d'Azur, CNRS, Institut Sophia Agrobiotech, Sophia-Antipolis, France.
PMID: 34285965 DOI: 10.21769/BioProtoc.2844
Abstract

Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA in plant tissues using a classical HPLC-based method.

Keywords

Arabidopsis thaliana; Defense hormone; HPLC; SA-glucoside; Salicylic acid.

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