2. Academic Validation
  3. Silver Nanoparticle-Coated Ethyl Cellulose Inhibits Tumor Necrosis Factor-α of Breast Cancer Cells

Silver Nanoparticle-Coated Ethyl Cellulose Inhibits Tumor Necrosis Factor-α of Breast Cancer Cells

  • Drug Des Devel Ther. 2021 May 13:15:2035-2046. doi: 10.2147/DDDT.S310760.
Ahmed A H Abdellatif 1 2,  Mansour Alsharidah 3,  Osamah Al Rugaie 4,  Hesham M Tawfeek 5,  Nahla Sameh Tolba 6
Affiliations

Affiliations

  • 1 Department of Pharmaceutics, College of Pharmacy, Qassim University, Buraydah, 51452, Saudi Arabia.
  • 2 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Assiut, 71524, Egypt.
  • 3 Department of Physiology, College of Medicine, Qassim University, Buraydah, 51452, Saudi Arabia.
  • 4 Department of Basic Medical Sciences, College of Medicine and Medical Sciences, Qassim University, Unaizah, AlQassim, 51911, Saudi Arabia.
  • 5 Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
  • 6 Department of Pharmaceutics, Faculty of Pharmacy, Sadat City University, Sadat City, Egypt.
PMID: 34012256 DOI: 10.2147/DDDT.S310760
Abstract

Introduction: Cancer is one of the leading causes of death worldwide. In many cases, Cancer is related to the elevated expression of a significant cytokine known as tumor necrosis factor-α (TNF-α). Breast Cancer in particular is linked to increased proliferation of tumor cells, high incidence of malignancies, more metastases, and generally poor prognosis for the patient. The research sought to assess the effect of silver nanoparticles reduced with ethyl cellulose polymer (AgNPs-EC) on TNF-α expression in MCF-7 human breast Cancer cells.

Methods: The AgNPs-EC were produced using the green synthesis reduction method, and their formation was proofed via UV-VIS spectroscopy. Furthermore, AgNPs-EC were characterized for their size, charge, morphology, Ag ion release, and stability. The MCF-7 cells were treated with AgNPs-EC. Then, the expression of TNF-α genes was determined through PCR in real time, and protein expression was studied using ELISA.

Results: The AgNPs-EC were spherical with an average size of 150±5.1 nm and a zeta-potential of -41.4±0.98 mV. AgNPs-EC had an inhibitory effect on cytokine mRNA and protein expression levels, which suggests that they could be used safely in the fight against Cancer. AgNPs-EC cytotoxicity was also found to be non-toxic to MCF-7.

Conclusion: Our data determined AgNPs-EC as a novel inhibitor of TNF-α production. These results are promising for developing novel therapeutic approaches for the future treatment of Cancer with safe Materials.

Keywords

MCF-7 cells; ethyl cellulose; silver nanoparticles; tumor necrosis factor-α.

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