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  3. Determination of butyrylcholinesterase activity based on thiamine luminescence modulated by MnO2 nanosheets

Determination of butyrylcholinesterase activity based on thiamine luminescence modulated by MnO2 nanosheets

  • Talanta. 2021 Mar 1:224:121831. doi: 10.1016/j.talanta.2020.121831.
Zhengyi Qu 1,  Tian Yu 2,  Yuzhong Liu 3,  Lihua Bi 4
Affiliations

Affiliations

  • 1 College of Chemistry, Jilin University, Changchun, 130012, PR China.
  • 2 College of Electrical and Electronic Engineering, Changchun University of Technology, Changchun, 130012, PR China.
  • 3 Jilin University First Hospital, Changchun, 130021, PR China.
  • 4 College of Chemistry, Jilin University, Changchun, 130012, PR China. Electronic address: blh@jlu.edu.cn.
PMID: 33379049 DOI: 10.1016/j.talanta.2020.121831
Abstract

In this paper, a novel strategy for biosensing butyrylcholinesterase (BChE) activity is developed based on manganese dioxide (MnO2) nanosheets to modulate the photoluminescence of thiamine (TH). The oxidase-like activity of MnO2 nanosheets enables them to catalyze the oxidation of non-fluorescent substrate TH to generate strong fluorescent thiochrome (TC). When the target BChE is introduced to form thiocholine in the presence of S-butyrylthiocholine iodide (BTCh), MnO2 nanosheets are reduced by thiocholine to Mn2+, resulting in the loss of their oxidase-like activity and the reduction of TC fluorescence. Based on this, a BChE activity fluorescence biosensor is constructed utilizing the luminescence behavior variation of TH and the oxidase-like activity of MnO2 nanosheets. The fluorescence biosensor shows a sensitive response to BChE, and the detection limit reaches 0.036 U L-1. In addition, the feasibility of the biosensor in real samples analysis is studied with satisfactory results.

Keywords

Butyrylcholinesterase; Enzymatic assay; Fluorescence change; Non-fluorescent substrate.

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