2. Academic Validation
  3. 3D-poly (lactic acid) scaffolds coated with gelatin and mucic acid for bone tissue engineering

3D-poly (lactic acid) scaffolds coated with gelatin and mucic acid for bone tissue engineering

  • Int J Biol Macromol. 2020 Nov 1:162:523-532. doi: 10.1016/j.ijbiomac.2020.06.157.
B Ashwin 1,  B Abinaya 1,  T P Prasith 1,  S Viji Chandran 1,  L Roshini Yadav 1,  M Vairamani 1,  Shantanu Patil 1,  N Selvamurugan 2
Affiliations

Affiliations

  • 1 Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, India.
  • 2 Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, India. Electronic address: selvamun@srmist.edu.in.
PMID: 32569692 DOI: 10.1016/j.ijbiomac.2020.06.157
Abstract

Three-dimensional (3D) printing is a promising technology to fabricate the intricate biomimetic structure. The primary focus of this study was to develop the bioactive 3D-scaffolds to enhance bone regeneration. The 3D-poly (lactic acid) (PLA) scaffolds were extruded based on a computer-aided design (CAD) model and coated with gelatin (Gel) containing different concentrations of mucic acid (MA) and were investigated for their osteogenic potential. Coating the PLA scaffolds with Gel/MA improved their physicochemical properties, and the addition of MA did not alter these properties. The viability of mouse mesenchymal stem cells (mMSCs, C3H10T1/2) seeded onto the PLA/Gel/MA scaffolds remained unaffected both at metabolic and cell membrane integrity levels. Alkaline Phosphatase and von Kossa staining indicated the promotion of osteoblast differentiation of mMSCs by MA in the PLA/Gel scaffolds. Inclusion of MA in PLA/Gel scaffolds also increased the expression of the master bone transcription factor, RUNX2, and Other osteoblastic differentiation marker genes in mMSCs. Thus, our results suggested that the 3D-printed PLA scaffolds coated with Gel/MA favor osteoblast differentiation and have potential applications in bone tissue engineering.

Keywords

3D-printing; Bone tissue engineering; Mucic acid; Poly(lactic acid); Runx2.

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