2. Academic Validation
  3. Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery

Avidin-biotin technology to synthesize multi-arm nano-construct for drug delivery

  • MethodsX. 2020 Apr 22:7:100882. doi: 10.1016/j.mex.2020.100882.
Chenzhen Zhang 1,  Tengfei He 1,  Armin Vedadghavami 1,  Ambika G Bajpayee 1 2
Affiliations

Affiliations

  • 1 Department of Bioengineering, Northeastern University, Boston, MA, 02115, USA.
  • 2 Department of Mechanical Engineering, Northeastern University, Boston, MA, 02115, USA.
PMID: 32405463 DOI: 10.1016/j.mex.2020.100882
Abstract

Here we describe methods for synthesizing a cationic, multi-arm Avidin (mAv) nano-construct that has a wide range of applications in drug delivery and imaging of negatively charged tissues. We use Avidin-biotin technology that gives the flexibility for conjugating biotinylated Dexamethasone to mAv by simple mixing at room temperature. We also describe methods to control hydrolysis rates of ester linkers to enable sustained (and tunable) drug release rates in therapeutic doses.•Multi-arm structure provides multiple sites for covalent conjugation of drugs•Use of Avidin-biotin reaction gives multi-arm nano-construct a modular design enabling conjugation and delivery of similar sized biotinylated drugs.

Keywords

Avidin; Biotin; Drug delivery; Ester linkers; Negatively charged tissues; PEGs.

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