2. Academic Validation
  3. c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice

c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice

  • J Agric Food Chem. 2020 Mar 25;68(12):3758-3769. doi: 10.1021/acs.jafc.0c00573.
Qing Ren 1 2,  Bo Yang 1 2,  Hao Zhang 1 2 3 4,  R Paul Ross 5 6,  Catherine Stanton 5 6 7,  Haiqin Chen 1 2,  Wei Chen 1 2 3 8
Affiliations

Affiliations

  • 1 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R China.
  • 2 School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R China.
  • 3 National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, P. R China.
  • 4 Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi, Jiangsu 214122, P. R China.
  • 5 International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, China.
  • 6 APC Microbiome Ireland, University College Cork, Cork T12 YN60, Ireland.
  • 7 Teagasc Food Research Centre, Moorepark, Fermoy, Cork P61 C996, Ireland.
  • 8 Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, P.R. China.
PMID: 32125157 DOI: 10.1021/acs.jafc.0c00573
Abstract

To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2). The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored. CLNA significantly inhibited weight loss, the disease activity index, and colon shortening. Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3). CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ. Moreover, CLNA increased the activity of oxidative stress-related Enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA. Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA. Furthermore, CLNA could rebalance the intestinal microbial composition of colitis mice, including increasing the α-diversity. CLNA1 and CLNA2 increased the abundance of Ruminococcus and Prevotella, respectively.

Keywords

colitis; conjugated linolenic acid (CLNA); gut microbiota; intestinal barrier function; oxidative stress.

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