2. Academic Validation
  3. Keth-seq for transcriptome-wide RNA structure mapping

Keth-seq for transcriptome-wide RNA structure mapping

  • Nat Chem Biol. 2020 May;16(5):489-492. doi: 10.1038/s41589-019-0459-3.
Xiaocheng Weng # 1 2,  Jing Gong # 3,  Yi Chen # 2,  Tong Wu # 1,  Fang Wang 1 4,  Shixi Yang 2,  Yushu Yuan 2,  Guanzheng Luo 1,  Kai Chen 1,  Lulu Hu 1,  Honghui Ma 1,  Pingluan Wang 1,  Qiangfeng Cliff Zhang 5,  Xiang Zhou 6,  Chuan He 7
Affiliations

Affiliations

  • 1 Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
  • 2 College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, China.
  • 3 MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
  • 4 Wuhan University School of Pharmaceutical Sciences, Wuhan, China.
  • 5 MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China. qczhang@tsinghua.edu.cn.
  • 6 College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, China. xzhou@whu.edu.cn.
  • 7 Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA. chuanhe@uchicago.edu.
  • # Contributed equally.
PMID: 32015521 DOI: 10.1038/s41589-019-0459-3
Abstract

RNA secondary structure is critical to RNA regulation and function. We report a new N3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine Bases in live cells. This N3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.

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