2. Academic Validation
  3. An "olivomycin A" derivative from a sponge-associated Streptomyces sp. strain SP 85

An "olivomycin A" derivative from a sponge-associated Streptomyces sp. strain SP 85

  • 3 Biotech. 2019 Dec;9(12):439. doi: 10.1007/s13205-019-1964-5.
Mohsen Gozari 1 2,  Nima Bahador 1,  Mohammad Seddiq Mortazavi 2,  Ebrahim Eftekhar 3,  Amir Reza Jassbi 4
Affiliations

Affiliations

  • 1 1Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
  • 2 2Persian Gulf and Oman Sea Ecological Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Bandar 'Abbas, Iran.
  • 3 3Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar 'Abbas, Iran.
  • 4 4Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
PMID: 31750037 DOI: 10.1007/s13205-019-1964-5
Abstract

We isolated an actinobacterium, Streptomyces sp. strain SP 85 from the marine Sponge Dysidea avara. Polyphasic identification of the microorganism showed that the strain SP 85 had high 16S rRNA gene similarity (99%) with Streptomyces olivaceus strain NBRC 12805, while some physiological and biochemical differences were observed. A cytotoxic compound, SP 85 was isolated from the active culture extract of the strain SP 85 by bioassay-guided purification over silica gel column chromatography, preparative TLC, and HPLC. The structure elucidation based on the spectroscopic analysis, including UV, ESI-MS, and 13C NMR data revealed that SP 85 compound is an analog of anti-tumor drug, "olivomycin A". The SP 85 compound showed high cytotoxic activity against three human Cancer cell lines, including SW480, HepG2, and MCF7 with IC50 values of 16, 93, and 78 nM, respectively. SP 85 exhibited significantly (2-10 times) higher cytotoxicity against the tumor cell lines in comparison with HUVECs as the normal cell line, which also induced Apoptosis in the tested cancerous cell line. This is the first report on the production of an "olivomycin A" derivative by a sponge-associated Streptomyces, showing the great potential of sponge-associated actinobacteria in producing cytotoxic Natural Products.

Keywords

Anti-tumor antibiotics; Dysidea avara; Olivomycin; Phylogenetic analysis; Sponge-associated Streptomyces.

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