2. Academic Validation
  3. Methyl Jasmonate Reduces Inflammation and Oxidative Stress in the Brain of Arthritic Rats

Methyl Jasmonate Reduces Inflammation and Oxidative Stress in the Brain of Arthritic Rats

  • Antioxidants (Basel). 2019 Oct 15;8(10):485. doi: 10.3390/antiox8100485.
Heloisa V Pereira-Maróstica 1,  Lorena S Castro 2,  Geferson A Gonçalves 3,  Francielli M S Silva 4,  Lívia Bracht 5,  Ciomar A Bersani-Amado 6,  Rosane M Peralta 7,  Jurandir F Comar 8,  Adelar Bracht 9,  Anacharis B Sá-Nakanishi 10
Affiliations

Affiliations

  • 1 Department of Biochemistry, University of Maringá, Maringá 7020900, Brazil. heloisavialle@gmail.com.
  • 2 Department of Biochemistry, University of Maringá, Maringá 7020900, Brazil. losacastro@hotmail.com.
  • 3 Department of Biochemistry, University of Maringá, Maringá 7020900, Brazil. gefersonag2@gmail.com.
  • 4 Department of Pharmacology and Therapeutics, University of Maringá, Maringá 87020900, Brazil. franciellimss@gmail.com.
  • 5 Department of Biochemistry, University of Maringá, Maringá 7020900, Brazil. lbracht@uem.br.
  • 6 Department of Pharmacology and Therapeutics, University of Maringá, Maringá 87020900, Brazil. cabamado@uem.br.
  • 7 Department of Biochemistry, University of Maringá, Maringá 7020900, Brazil. rmperalta@uem.br.
  • 8 Department of Biochemistry, University of Maringá, Maringá 7020900, Brazil. jfcomar@uem.br.
  • 9 Department of Biochemistry, University of Maringá, Maringá 7020900, Brazil. abracht@uem.br.
  • 10 Department of Biochemistry, University of Maringá, Maringá 7020900, Brazil. absnakanishi@uem.br.
PMID: 31618993 DOI: 10.3390/antiox8100485
Abstract

Methyl jasmonate (MeJA), common in the plant kingdom, is capable of reducing articular and hepatic inflammation and oxidative stress in adjuvant-induced arthritic rats. This study investigated the actions of orally administered MeJA (75-300 mg/kg) on inflammation, oxidative stress and selected enzyme activities in the brain of Holtzman rats with adjuvant-induced arthritis. MeJA prevented the arthritis-induced increased levels of nitrites, nitrates, lipid peroxides, protein carbonyls and Reactive Oxygen Species (ROS). It also prevented the enhanced activities of myeloperoxidase and Xanthine Oxidase. Conversely, the diminished catalase and superoxide dismutase activities and glutathione (GSH) levels caused by arthritis were totally or partially prevented. Furthermore, MeJA increased the activity of the mitochondrial isocitrate dehydrogenase, which helps to supply NADPH for the mitochondrial glutathione cycle, possibly contributing to the partial recovery of the GSH/oxidized glutathione (GSSG) ratio. These positive actions on the antioxidant defenses may counterbalance the effects of MeJA as enhancer of ROS production in the mitochondrial respiratory chain. A negative effect of MeJA is the detachment of Hexokinase from the mitochondria, which can potentially impair glucose phosphorylation and metabolism. In overall terms, however, it can be concluded that MeJA attenuates to a considerable extent the negative effects caused by arthritis in terms of inflammation and oxidative stress.

Keywords

adjuvant-induced arthritis; brain mitochondria; hexokinase; methyl jasmonate; oxidative stress; rheumatoid arthritis.

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