2. Academic Validation
  3. Wound healing activity of terpinolene and α-phellandrene by attenuating inflammation and oxidative stress in vitro

Wound healing activity of terpinolene and α-phellandrene by attenuating inflammation and oxidative stress in vitro

  • J Tissue Viability. 2019 May;28(2):94-99. doi: 10.1016/j.jtv.2019.02.003.
Marcella Malavazi de Christo Scherer 1,  Franciane Martins Marques 1,  Mariana Moreira Figueira 1,  Maria Carolina Oliveira Peisino 1,  Elisângela Flávia Pimentel Schmitt 1,  Tamara P Kondratyuk 2,  Denise Coutinho Endringer 1,  Rodrigo Scherer 1,  Marcio Fronza 3
Affiliations

Affiliations

  • 1 Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Produtos Naturais, Universidade Vila Velha, CEP 29102-920, Brazil.
  • 2 Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI, 96720, USA.
  • 3 Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Produtos Naturais, Universidade Vila Velha, CEP 29102-920, Brazil. Electronic address: marcio.fronza@uvv.br.
PMID: 30792116 DOI: 10.1016/j.jtv.2019.02.003
Abstract

This study was undertaken to investigate the in vitro wound healing effects and the anti-inflammatory and antioxidant activities of terpinolene and α-phellandrene. The in vitro stimulatory effects on the proliferation and migration of fibroblasts were assessed using the scratch assay. The anti-inflammatory activity was evaluated using cell-based assays by investigating their influence on nitric oxide (NO), superoxide anion (O2•-), tumour necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) production and using the TNF-α-induced nuclear factor kappa (NF-κB) assay. Antioxidant activity was determined by the ABTS cation radical scavenging capacity, ferric reducing/antioxidant potential (FRAP), and NO free radical scavenging assays. Terpinolene and α-phellandrene significantly increased the migration and proliferation of fibroblasts and suppressed the pro-inflammatory cytokines IL-6 and TNF-α in a dose-dependent manner. Terpinolene and α-phellandrene at a concentration of 100 μM significantly inhibited NO production (41.3 and 63.8%, respectively) in a macrophage cell-culture-based assay, and resulted in reductions in O2•- production of 82.1 ± 3.5% and 70.6 ± 4.3%, respectively. Moreover, these monoterpenes were verified to suppress NF-κB activity. In summary, terpinolene and α-phellandrene may contribute to broadening clinical options in the treatment of wounds by attenuating inflammation and oxidative stress in vitro.

Keywords

Cytokines; Inflammation; Migration; Scratch assay; Wound healing.

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