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  3. Methods and Current Trends in Determination of Neuraminidase Activity and Evaluation of Neuraminidase Inhibitors

Methods and Current Trends in Determination of Neuraminidase Activity and Evaluation of Neuraminidase Inhibitors

  • Crit Rev Anal Chem. 2019;49(4):350-367. doi: 10.1080/10408347.2018.1531692.
Zuzana Hľasová 1,  Ivan Košík 2,  Miroslav Ondrejovič 1,  Stanislav Miertuš 1 3,  Jaroslav Katrlík 4
Affiliations

Affiliations

  • 1 a Department of Biotechnology , Faculty of Natural Sciences of University Ss. Cyril and Methodius , Trnava , Slovakia.
  • 2 b Cellular Biology Section, Laboratory of Viral Diseases , NIAID , Bethesda , Maryland , USA.
  • 3 c International Centre for Applied Research and Sustainable Technology , Bratislava , Slovakia.
  • 4 d Department of Glycobiotechnology , Institute of Chemistry, Slovak Academy of Sciences , Bratislava , Slovakia.
PMID: 30582732 DOI: 10.1080/10408347.2018.1531692
Abstract

This review focuses on the methods and current trends in determination of neuraminidases (NAs) activity and evaluation of neuraminidase inhibitors (NAIs) by means of biochemical assays. These methods can be used, in principle, for any type of sialidase, with regard to substrate specificity and optimal conditions for enzymatic reaction. Considering the range of organisms producing sialidases, this review omits cell-based assays (plaque assays and study of cytopathic effect) and animal model studies, which are reviewed elsewhere concerning specific organisms. The present review also provides the brief introductory survey of role of sialic acids and neuraminidases, but main focus is on the methods of determining neuraminidase activity and evaluating neuraminidase inhibitors. The future prospect of improvement in analytical techniques regarding the enzymatic activity of sialidases is briefly outlined.

Keywords

Bioanalytical; enzyme-based assay; lectin; neuraminidase; sialic acid.

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