2. Academic Validation
  3. Dual-functional lipid-like nanoparticles for delivery of mRNA and MRI contrast agents

Dual-functional lipid-like nanoparticles for delivery of mRNA and MRI contrast agents

  • Nanoscale. 2017 Jan 26;9(4):1575-1579. doi: 10.1039/c6nr08496f.
X Luo 1,  B Li 1,  X Zhang 1,  W Zhao 1,  A Bratasz 2,  B Deng 3,  D W McComb 3,  Y Dong 1
Affiliations

Affiliations

  • 1 Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA. dong.525@osu.edu.
  • 2 Small Animal Imaging Center, The Ohio State University, Columbus, Ohio 43210, USA.
  • 3 Center for Electron Microscopy and Analysis, Department of Materials Science and Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
PMID: 28067926 DOI: 10.1039/c6nr08496f
Abstract

Multi-functional nanomaterials possess unique properties, facilitating both therapeutic and diagnostic applications among Others. Herein, we developed dual-functional lipid-like nanoparticles for simultaneous delivery of mRNA and magnetic resonance imaging (MRI) contrast agents in order to express functional proteins and provide real-time visualization. TT3-Gd18 LLNs were identified as a lead formulation, which was able to encapsulate 91% of mRNA and 74% of Gd. This formulation showed a comparable or a slightly higher delivery efficiency of mRNA compared to the initial TT3 LLNs. Moreover, a strong MRI signal was observed in the cell pellets treated with TT3-Gd18 LLNs. More importantly, TT3-Gd18 LLNs demonstrated an efficient delivery of mRNA and Gd contrast agents in vivo.

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