2. Academic Validation
  3. Vermelhotin, an anti-inflammatory agent, suppresses nitric oxide production in RAW 264.7 cells via p38 inhibition

Vermelhotin, an anti-inflammatory agent, suppresses nitric oxide production in RAW 264.7 cells via p38 inhibition

  • J Nat Prod. 2013 Sep 27;76(9):1824-7. doi: 10.1021/np400565e.
Acharavadee Pansanit 1,  Eun-Jung Park,  Tamara P Kondratyuk,  John M Pezzuto,  Kriengsak Lirdprapamongkol,  Prasat Kittakoop
Affiliations

Affiliation

  • 1 Chemical Biology Program, Chulabhorn Graduate Institute , Kamphaeng Phet 6 Road, Laksi, Bangkok 10210, Thailand.
PMID: 24016057 DOI: 10.1021/np400565e
Abstract

Vermelhotin exhibited potential anti-inflammatory activity through inhibition of nitric oxide production (IC₅₀ = 5.35 ± 0.59 μM) in LPS-stimulated RAW 264.7 macrophage cells. Vermelhotin suppressed expression of inducible nitric oxide synthase (iNOS) at mRNA and protein levels, in a dose-dependent manner. Mechanistic studies revealed that vermelhotin abrogated upstream signaling of iNOS expression by selectively inhibiting p38 phosphorylation, while ERK and JNK activations were not affected.

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