2. Academic Validation
  3. Probing single-stranded DNA and its biomolecular interactions through direct catalytic activation of factor XII, a protease of the blood coagulation cascade

Probing single-stranded DNA and its biomolecular interactions through direct catalytic activation of factor XII, a protease of the blood coagulation cascade

  • Biochem Biophys Res Commun. 2006 Oct 27;349(3):1011-5. doi: 10.1016/j.bbrc.2006.08.156.
Valeri Pavlov 1,  Michael Zorn,  Roland Krämer
Affiliations

Affiliation

  • 1 Anorganisch-Chemisches Institut, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.
PMID: 16970924 DOI: 10.1016/j.bbrc.2006.08.156
Abstract

Triggered self-activation of factor XII, a blood coagulation protease, was utilized for the amplified visual detection of ss-DNA targets in a non-sequence specific way. Factor XII holds potential as a low-affinity and therefore non-interfering probe for DNA secondary structure and for the screening of protein binding to ss-DNA. The observation that ss-DNA also accelerates coagulation of human blood plasma is relevant to the emerging field of aptamer therapeutics.

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