2. Academic Validation
  3. Characterization and biological activity of the main flavonoids from Swiss Chard (Beta vulgaris subspecies cycla)

Characterization and biological activity of the main flavonoids from Swiss Chard (Beta vulgaris subspecies cycla)

  • Phytomedicine. 2007 Feb;14(2-3):216-21. doi: 10.1016/j.phymed.2006.03.006.
P Ninfali 1,  M Bacchiocca,  A Antonelli,  E Biagiotti,  A M Di Gioacchino,  G Piccoli,  V Stocchi,  G Brandi
Affiliations

Affiliation

  • 1 Istituto di Chimica Biologica G. Fornaini, Università degli Studi Carlo Bo, Urbino (PU), Italy. p.ninfali@uniurb.it
PMID: 16698256 DOI: 10.1016/j.phymed.2006.03.006
Abstract

The molecular components of a phenolic fraction (P2), obtained from liquid chromatography of a Swiss Chard (Beta vulgaris subsp. cycla) extract, were identified using HPLC-ESI-MS/MS. The primary P2 components were: vitexin-2''O-rhamnoside, its demethylated form 2''-xylosylvitexin, isorhamnetin 3-gentiobioside, and rutin. P2 "in toto" and the single components were characterized for antioxidant capacity, antimitotic activity on MCF-7 human breast Cancer cells and for toxicity to human lymphocytes and macrophages. P2 inhibited MCF-7 cell proliferation (IC(50) value = 9 microg/ml) without inducing Apoptosis, showed no toxicity to human lymphocytes and slight toxicity to macrophages. Vitexin-2''O-rhamnoside strongly inhibited DNA synthesis in MCF-7 cells, whereas 2''-xylosylvitexin and isorhamnetin 3-gentiobioside were activators; combinations of activators and inhibitors maintained the over-all inhibitory effect.

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