Ligustroside (Synonyms: Ligstroside)

Name: Ligustroside
Brand: MedChemExpress
SKU: HY-N3383
Rating: 4.5 (1 reviews)

Cat. No.: HY-N3383 Purity: 99.75%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Ligustroside (Ligstroside), a secoiridoid derivative, has outstanding performance on mitochondrial bioenergetics in models of early Alzheimer's disease (AD) and brain ageing by mechanisms that may not interfere with Aβ production. Ligustroside significantly inhibits nitric oxide production in lipopolysaccharide-activated RAW264.7 macrophages.

For research use only. We do not sell to patients.

Ligustroside Chemical Structure

CAS No. : 35897-92-8

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
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10 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
SK-BR-3	IC₅₀	> 20 μM Compound: 9	Antiproliferative activity against human SKBR3 cells after 72 hrs by MTT reduction assay Antiproliferative activity against human SKBR3 cells after 72 hrs by MTT reduction assay	[PMID: 28551177]

In Vitro

Ligstroside increases mRNA expression of SIRT1, CREB1, complex I, and GPx1 in SH-SY5Y-APP₆₉₅ cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ligstroside (50 mg/kg; a supplemented diet for 6 months) protects against mitochondrial dysfunction in models of early Alzheimer's disease and brain ageing^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female NMRI mice, aged 12 months^[1]
Dosage:	50 mg/kg
Administration:	Received a supplemented diet for 6 months (equivalent to 6.25 mg/kg b.w.)
Result:	Showed improved spatial working memory; Restored brain ATP levels in aged mice and led to a significant life extension compared to aged control animals.

Molecular Weight

524.51

Formula

C₂₅H₃₂O₁₂

CAS No.

35897-92-8

Appearance

Solid

Color

White to off-white

SMILES

OC(C=C1)=CC=C1CCOC(C[C@H]2/C([C@@H](OC=C2C(OC)=O)O[C@@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)=C\C)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (190.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9065 mL	9.5327 mL	19.0654 mL
5 mM	0.3813 mL	1.9065 mL	3.8131 mL
10 mM	0.1907 mL	0.9533 mL	1.9065 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.77 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (250 KB) HNMR (284 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Grewal R, et al. Purified oleocanthal and ligstroside protect against mitochondrial dysfunction in models of early Alzheimer's disease and brain ageing. Exp Neurol. 2020;328:113248. [Content Brief]

[2]. Liu J, et al. Chemical structures of constituents from the flowers of Osmanthus fragrans var. aurantiacus. J Nat Med. 2015;69(1):135-141. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9065 mL	9.5327 mL	19.0654 mL	47.6635 mL
	5 mM	0.3813 mL	1.9065 mL	3.8131 mL	9.5327 mL
	10 mM	0.1907 mL	0.9533 mL	1.9065 mL	4.7664 mL
	15 mM	0.1271 mL	0.6355 mL	1.2710 mL	3.1776 mL
	20 mM	0.0953 mL	0.4766 mL	0.9533 mL	2.3832 mL
	25 mM	0.0763 mL	0.3813 mL	0.7626 mL	1.9065 mL
	30 mM	0.0636 mL	0.3178 mL	0.6355 mL	1.5888 mL
	40 mM	0.0477 mL	0.2383 mL	0.4766 mL	1.1916 mL
	50 mM	0.0381 mL	0.1907 mL	0.3813 mL	0.9533 mL
	60 mM	0.0318 mL	0.1589 mL	0.3178 mL	0.7944 mL
	80 mM	0.0238 mL	0.1192 mL	0.2383 mL	0.5958 mL
	100 mM	0.0191 mL	0.0953 mL	0.1907 mL	0.4766 mL

Ligustroside Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ligustroside35897-92-8LigstrosideMitochondrial Metabolismsecoiridoid glucosideAlzheimer's diseasemitochondrial dysfunctionbrainageingInhibitorinhibitorinhibit

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Ligustroside (Synonyms: Ligstroside)

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Ligustroside Related Antibodies

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Complete Stock Solution Preparation Table

Ligustroside Related Classifications

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