2. Apoptosis Cell Cycle/DNA Damage
  3. Ferroptosis Polo-like Kinase (PLK) MDM-2/p53
  4. L14-8

L14-8 is a potent ferroptosis inducer. L14-8 promotes PLK1 degradation via ubiquitination, increasing TP53 phosphorylation to enhance SAT1 transcription, thereby triggering ferroptosis and cancer cell death. DNH28 can be used for the study of advanced prostate cancer.

For research use only. We do not sell to patients.

L14-8 Chemical Structure

L14-8 Chemical Structure

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L14-8 Related Antibodies

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Description

L14-8 is a potent ferroptosis inducer. L14-8 promotes PLK1 degradation via ubiquitination, increasing TP53 phosphorylation to enhance SAT1 transcription, thereby triggering ferroptosis and cancer cell death. DNH28 can be used for the study of advanced prostate cancer[1].

In Vitro

L14-8 (5-25 μM, 48 h) results in over 80% cell death in both C4-2B and 22Rv1 cells at 25 μM and does not significantly affect normal prostate cell growth[1].
L14-8 (10 μM, 24-48 h) induces ferroptosis by transcriptionally activating SAT1 expression and increase MDA level in C4-2B and 22Rv1 cells[1].
L14-8 (0-10 μM, 0-24 h) binds to PLK1 and enhances PLK1-mediated TP53 phosphorylation and expression, which is required for SAT1 transcription activation in C4-2B and 22Rv1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L14-8 Related Antibodies

Cell Viability Assay[1]

Cell Line: C4-2B and 22Rv1 cells
Concentration: 5 and 7.5 μM
Incubation Time: 72 h
Result: Significantly rescued the inhibitory effect of L14-8 on the viability of C4-2B and 22Rv1 cells.
Significantly reduced the sensitivity of the cells after knocking out TP53.

RT-PCR[1]

Cell Line: C4-2B and 22Rv1 cells
Concentration: 10 μM
Incubation Time: 48 h
Result: Dramatically increased the the expression of ferroptosis-related genes SAT1.

Western Blot Analysis[1]

Cell Line: C4-2B and 22Rv1 cells
Concentration: 0, 1, 2, 4, 8, 12 and 24 h
Incubation Time: 72 h
Result: Significantly increased the thermal stability of endogenous PLK1.
led to an increase in the protein levels of TP53 and phosphorylated TP53 (p-TP53) .
Significantly shortened the half-life of PLK1.
In Vivo

L14-8 (10-20 mg/kg, i.p., once daily for 25 days) suppresses prostate cancer growth without significant toxicity in C4-2B xenograftd mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C4-2B cells xenografted model established in male 4-week-old BALB/c nude mice[1]
Dosage: 10 and 20 mg/kg
Administration: Intraperitoneal injection (i.p.), once daily for 25 days
Result: Significantly suppressed tumor growth in a dose-dependent manner.
Decreased cancer proliferation.
Had no changes in major organs such as the heart, kidney, liver, lung, and spleen with high doses.
Had similar liver and kidney function indices between vehicle treatment in lower doses.
Molecular Weight

517.52

Formula

C30H25F2NO5
SMILES

FC1=CC=C([C@@H](OC(C=C)=O)CC[C@@H]2[C@@H](C3=CC=C(OC(C=C)=O)C=C3)N(C4=CC=C(F)C=C4)C2=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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L14-8 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L14-8FerroptosisPolo-like Kinase (PLK)MDM-2/p53SAT1TP53Drug design and optimizationProstate cancerInhibitorinhibitorinhibit

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