2. Cell Cycle/DNA Damage
  3. Checkpoint Kinase (Chk)
  4. K1586

K1586 is an amidine derivative that efficiently targets Chk1. K1586 enhances the degradation of Chk1 that sensitizes colorectal cancer cells to ionizing radiation. K1586 shows anticancer effects.

For research use only. We do not sell to patients.

K1586

K1586 Chemical Structure

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Based on 1 publication(s) in Google Scholar

K1586 Related Antibodies

Top Publications Citing Use of Products

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Chk1 Chk2 BUBR1

  • Biological Activity

  • Purity & Documentation

  • References

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Description

K1586 is an amidine derivative that efficiently targets Chk1. K1586 enhances the degradation of Chk1 that sensitizes colorectal cancer cells to ionizing radiation. K1586 shows anticancer effects[1].

In Vitro

K1586 (0.001-100 μM; 72 h) decreases cell proliferation in a dose-dependent manner[1].
K1586 (10-20 μM; 24 h) increases the relative length of the S phase and significant increases the length of the G2/M phase[1].
K1586 (10 μM; 12 h) decreases Chk1 protein levels in a time-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

K1586 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: HT29 human colorectal cancer cells
Concentration: 0.001 μM, 0.01 μM, 0.1 μM, 1 μM, 5 μM, 10 μM, 50 μM, and 100 μM
Incubation Time: 72 h
Result: Proliferation decreased in a dose-dependent manner.

Cell Cycle Analysis[1]

Cell Line: HT29 cells
Concentration: 10 or 20 μM
Incubation Time: 24 h
Result: Increased the length of the G2/M phase in cells.

Western Blot Analysis[1]

Cell Line: HT29 cells
Concentration: 10 μM
Incubation Time: 12 h
Result: Induced Chk1 destabilization and degradation.
Molecular Weight

359.87

Formula

C18H18ClN3OS
Appearance

Solid

Color

White to off-white

SMILES

CCN(CC)/C=N/C(C=C1)=CC2=C1N=C(S2)OC3=CC=C(Cl)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (138.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7788 mL 13.8939 mL 27.7878 mL
5 mM 0.5558 mL 2.7788 mL 5.5576 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7788 mL 13.8939 mL 27.7878 mL 69.4695 mL
5 mM 0.5558 mL 2.7788 mL 5.5576 mL 13.8939 mL
10 mM 0.2779 mL 1.3894 mL 2.7788 mL 6.9470 mL
15 mM 0.1853 mL 0.9263 mL 1.8525 mL 4.6313 mL
20 mM 0.1389 mL 0.6947 mL 1.3894 mL 3.4735 mL
25 mM 0.1112 mL 0.5558 mL 1.1115 mL 2.7788 mL
30 mM 0.0926 mL 0.4631 mL 0.9263 mL 2.3157 mL
40 mM 0.0695 mL 0.3473 mL 0.6947 mL 1.7367 mL
50 mM 0.0556 mL 0.2779 mL 0.5558 mL 1.3894 mL
60 mM 0.0463 mL 0.2316 mL 0.4631 mL 1.1578 mL
80 mM 0.0347 mL 0.1737 mL 0.3473 mL 0.8684 mL
100 mM 0.0278 mL 0.1389 mL 0.2779 mL 0.6947 mL
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K1586 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

K1586K 1586K-1586Checkpoint Kinase (Chk)anticancer agentChk1 inhibitorionizing radiationamidine derivativeInhibitorinhibitorinhibit

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