2. JAK/STAT Signaling
  3. Pim
  4. K00135

K00135 is a potent and selective inhibitor of PIM kinases. K00135 inhibits survival and clonogenic growth of acute leukemia cells. K00135 inhibits the phosphorylation of PIM downstream targets.

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K00135

K00135 Chemical Structure

CAS No. : 869650-21-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
Solid
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K00135 Related Antibodies

Top Publications Citing Use of Products

View All Pim Isoform Specific Products:

View All Isoforms
PIM1 PIM2 PIM3

  • Biological Activity

  • Purity & Documentation

  • References

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Description

K00135 is a potent and selective inhibitor of PIM kinases. K00135 inhibits survival and clonogenic growth of acute leukemia cells. K00135 inhibits the phosphorylation of PIM downstream targets[1].

Cellular Effect
Cell Line Type Value Description References
K562 IC50
8 μM
Compound: K00135
Growth inhibition of human K562 cells after 48 hrs by WST-1 assay
Growth inhibition of human K562 cells after 48 hrs by WST-1 assay
[PMID: 22136433]
MOLM-13 IC50
0.3 μM
Compound: K00135
Growth inhibition of human MOLM13 cells after 48 hrs by WST-1 assay
Growth inhibition of human MOLM13 cells after 48 hrs by WST-1 assay
[PMID: 22136433]
MV4-11 IC50
0.4 μM
Compound: K00135
Growth inhibition of human MV4-11 cells after 48 hrs by WST-1 assay
Growth inhibition of human MV4-11 cells after 48 hrs by WST-1 assay
[PMID: 22136433]
RS4-11 IC50
8.8 μM
Compound: K00135
Growth inhibition of human RS4:11 cells after 48 hrs by WST-1 assay
Growth inhibition of human RS4:11 cells after 48 hrs by WST-1 assay
[PMID: 22136433]
SEM IC50
6 μM
Compound: K00135
Growth inhibition of human SEM cells after 48 hrs by WST-1 assay
Growth inhibition of human SEM cells after 48 hrs by WST-1 assay
[PMID: 22136433]
Molecular Weight

306.36

Formula

C18H18N4O
CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

CC(C1=CC=CC(C2=CN=C3C=CC(NCC4CC4)=NN32)=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 11.67 mg/mL (38.09 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2641 mL 16.3207 mL 32.6413 mL
5 mM 0.6528 mL 3.2641 mL 6.5283 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2641 mL 16.3207 mL 32.6413 mL 81.6033 mL
5 mM 0.6528 mL 3.2641 mL 6.5283 mL 16.3207 mL
10 mM 0.3264 mL 1.6321 mL 3.2641 mL 8.1603 mL
15 mM 0.2176 mL 1.0880 mL 2.1761 mL 5.4402 mL
20 mM 0.1632 mL 0.8160 mL 1.6321 mL 4.0802 mL
25 mM 0.1306 mL 0.6528 mL 1.3057 mL 3.2641 mL
30 mM 0.1088 mL 0.5440 mL 1.0880 mL 2.7201 mL
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K00135 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

K00135869650-21-5K 00135K-00135PimPim kinasesAntileukemicPIM kinasesacute myelogenous leukemiaAMLInhibitorinhibitorinhibit

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