Jun6504 

Jun6504 is an enterovirus 2C inhibitor. Jun6504 shows potent and broad-spectrum antiviral activity against multiple strains of EV-D68 (EC₅₀ = 250 nM), EV-A71 (EC₅₀ = 502.4 nM), and CVB3 (EC₅₀ = 1049 nM). Jun6504 improves paralysis score and weight gain in a neonatal mouse model of EV-D68 infection. Jun6504 reduces viral titers in the spinal cord and the infected quadriceps muscle. Jun6504 can be used for EV-D68 antiviral research^[1].

Jun6504 (1-10000 nM) shows the antiviral activity EC₅₀ values of 250 nM (EV-D68), 502.4 nM (EV-A71) and 1049 nM (CVB3) in RD cells^[1].
Jun6504 (1-10000 nM, 60 h) shows antiviral activity against five strains of EVD68 (US/MO114-18947, USMO114-18949, USIL/14-18952, US/L14-18956, USKY114-18953) with EC₅₀ values ranging from 0.25 to 0.47 µM in RD cells^[1].
Jun6504 (0.01-10 μM, 3 days) exhibits dose-dependent inhibition against CVA16, CVA6, poliovirus type 1, and CVB3 in CPE assay with EC₅₀ values from 0.24 to 0.57 µM in CPE assay and inhibits EV-A71 and EV-D68 plaque formation in RD cells with EC₅₀ values of 0.22 and 0.13 µM, respectively^[1].
Jun6504 (1-1000 μM) displays dose-dependent increases in melting temperatures of the EV-A71, EV-D68, and CVB3 2C proteins^[1].
Jun6504 blocks viral replication at an intermediate stage in the viral life cycle evidenced by inhibiting EV-D68 amplification when adds before or at 3 h post-infection (hpi), an effect that can be inhibited when adds at 5 hpi and 7 hpi^[1].
Jun6504 (12.8-100 μM) shows the EC₅₀s against P3, P6, and P9 viruses are determined in RD cells as 0.80, 3.38, and 15.45 μM, respectively and shows complete loss of antiviral activity against P7 and P10 viruses, with EC₅₀ values exceeding 30 µM^[1].
Jun6504 (0.001-10 μM) exhibits the weakest activity against r2C-I112V/D183V/D323G, with an EC₅₀ of 3.77 µM and shows complete loss of antiviral activity against the EV-D68 2C mutant proteins (F190L, D183V/F190L, D183V/F190L/D323G)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Jun6504 (50 mg/kg, i.p., once daily, 13-14 days) improves paralysis score and weight gain in a neonatal mouse model of EV-D68 infection^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

327.42

C₁₈H₂₅N₅O

O=C(NCC1(CNC1)C)C2=C(C=NN3C(C)C)C3=NC(C4CC4)=C2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Li K, et al. A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis. Nat Commun. 2025 Jul 1;16(1):5987.  [Content Brief]