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  2. Protein Biology
  3. Protein Purification
  4. Agarose Beads
  5. Protein A Agarose (Alkali-Tolerant) 4FF

Protein A Agarose (Alkali-Tolerant) 4FF 

Cat. No.: HY-K0251
Manual COA SDS Technical Support

MCE Protein A Agarose (Alkali-Tolerant) 4FF can efficiently binds IgG from serum, ascites, culture supernatants, and other antibody-containing samples, enabling high-performance antibody purification.

Protein A Agarose (Alkali-Tolerant) 4FF
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  • Description

  • Storage

  • Attention

  • Documentation

Description
& Advantages

Protein A is a cell wall protein originally isolated from Staphylococcus aureus, known for its specific binding to the Fc region of mammalian immunoglobulin G (IgG) heavy chains. Native protein A contains five IgG-binding domains, along with several additional domains of unknown function. In contrast, recombinant protein A retains only the IgG-binding domains, significantly reducing nonspecific adsorption.

MCE Protein A Agarose (Alkali-Tolerant) 4FF is manufactured by covalently coupling recombinant protein A to a highly cross-linked agarose matrix. It features high binding capacity, excellent specificity, and strong ligand stability. The resin is alkali-tolerant and can withstand in-place cleaning with 0.5 M NaOH, making it suitable for repeated use. This product efficiently binds IgG from serum, ascites, culture supernatants, and other antibody-containing samples, enabling high-performance antibody purification.

Each 1 mL of total volume contains 0.5 mL of resin. Thoroughly resuspend the resin before use and mix well before aliquoting.

 

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

Storage

4°C, 5 years.

Do not dry or freeze.

Attention

1. Ensure the resin is thoroughly resuspended before use.

2. It is recommended to replace the storage buffer every 2-3 months to prevent microbial contamination due to ethanol evaporation.

3. To minimize protein degradation, protease inhibitor cocktails (MCE Cat. No. HY-K0010, HY-K0011) are highly recommended.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.

Documentation

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Protein A Agarose (Alkali-Tolerant) 4FF
Cat. No.:
HY-K0251
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