Mouse Airway Organoid Kit 

MCE Mouse Airway Organoid Kit includes a basic culture medium for Mouse Airway organoids, as well as culture supplements, and is designed for the establishment and long-term maintenance of mouse airway organoids derived from airway stem cells.

The self-renewal of the airway epithelium is primarily driven by the proliferation of basal stem cells. Mouse airway organoids generated using this kit contain a diverse array of epithelial cell types, including basal cells, ciliated cells, secretory cells, and a small population of neuroendocrine cells. As such, these organoids faithfully recapitulate the architecture, cellular composition, and dynamic self-renewal properties of the native airway epithelium. This makes them a powerful model for studying airway development, regeneration, and disease mechanisms.

Basal Medium A: 4°C, 1 year. Shipping with blue ice.

Supplement B (50×) & Supplement C (250×): -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry a ice.

1. Cells need to be kept sterile when extracted from primary tissue to avoid contamination from subsequent experiments.

2. Observe the fragmentation status of the organoid during passaged dissociation, and terminate the dissociation when small cell clusters (10-50 cells) appear to avoid prolonging the subsequent growth viability of the organoid.

3. Operations involving MCE Basement Membrane Matrix need to be kept at low temperature throughout. MCE Basement Membrane Matrix should be injected rapidly into the cell culture wells after resuspension with the cells, while avoiding air bubbles.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.