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  3. Cell Biology
  4. Cell Culture
  5. Cell Transfection
  6. Lentivirus Transfection Reagent

Lentivirus Transfection Reagent 

Cat. No.: HY-K2015
Manual COA SDS Technical Support

MCE Lentivirus Transfection Reagent is a new type of transfection reagent based on cationic polymer, which is suitable for lentivirus packaging and transfection. It significantly improves the efficiency of packaging and transfection, resulting in the production of more recombinant lentivirus.

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1 Publications Citing Use of MCE Lentivirus Transfection Reagent

    MedChemExpress Validation
    Conditions: 75% cell density for lentivirus packaging; 4 μg GFP control plasmid + 4 μL helper mix + 40 μL transfection reagent
    Detection: Collect supernatant at 48 h post-transfection, add 10 mL fresh medium; re-collect at 72 h, mix and concentrate. Titer tested in HEK293 via well dilution. After 16 h infection, change medium; culture 48 h, observe GFP under fluorescence microscope
    HY-K2015 enabled efficient lentivirus transfection with high titer of packaged control plasmid virus.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    Lentivirus vector is a gene therapy vector based on HIV-1, which can stably integrate exogenous genes into the host cell genome. It offers the advantages such as an extensive infection spectrum, effective infection and division period, static cell cells, long-term stable expression of exogenous genes, etc. It has become a powerful tool for importing exogenous genes, and has been widely used in gene overexpression in various cell lines, RNA interference, microRNA research and in vivo animal experiments.

    MCE Lentivirus Transfection Reagent is a new type of transfection reagent based on cationic polymer, which is suitable for lentivirus packaging and transfection. It significantly improves the efficiency of packaging and transfection, resulting in the production of more recombinant lentivirus. The viral titer of the control plasmid obtained by transfection and packaging can reach 108 TU/mL, which has the advantages of high infection efficiency, wide infection spectrum, high reproducibility and simple operation.
    Storage

    -20℃, 1 year

    Avoid repeat freeze-thaw cycles.
    Components
    Components HY-K2015-1 mL HY-K2015-5 mL
    Lentivirus Transfection Reagent 1 mL 1 mL × 5
    Documentation

    Manual (1578 KB) SDS (342 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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